CD3D Antibody

Overview

This is a polyclonal anti-CD3D antibody raised in Rabbit, with confirmed utility in WB, ELISA. It is designed to detect CD3D protein in Human and supports researchers working in immunology contexts where consistent antibody performance is required.

Key elements and design rationale

• Immunogen: Synthesized peptide derived from the Internal region of Human CD3-δ. — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.
• Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.
• Polyclonal format: Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.
• Isotype (IgG): Compatible with standard Protein A/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.
• Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.

Biological background

CD3D (also referred to as 4930549J05Rik, A430104F18Rik, AW552088, Cd247, CD247 antigen, CD247 antigen, zeta subunit, CD247 molecule, CD3, CD3 antigen, delta subunit, CD3 delta, CD3 epsilon, CD3 eta, CD3 gamma, CD3 molecule delta polypeptide, CD3 molecule, epsilon polypeptide, CD3 molecule, gamma polypeptide, CD3 zeta, CD3-DELTA, CD3d, CD3D antigen delta polypeptide, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule delta, CD3d molecule delta CD3 TCR complex, CD3d molecule, delta (CD3-TCR complex), CD3D_HUMAN, CD3E, CD3e antigen, CD3E antigen epsilon polypeptide, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule epsilon, CD3e molecule epsilon CD3 TCR complex, CD3e molecule, epsilon (CD3-TCR complex), CD3epsilon, CD3G, CD3g antigen, CD3G antigen gamma polypeptide, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule gamma, CD3g molecule gamma CD3 TCR complex, CD3g molecule, gamma (CD3-TCR complex), CD3H, CD3Q, CD3Z, CD3zeta, Ctg3, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD19, Leu-4, MGC138597, OKT3, delta chain, OTTHUMP00000032544, T cell receptor, T cell receptor T3 delta chain, T cell receptor T3 gamma chain, T cell receptor T3 zeta chain, T cell receptor zeta chain, T cell surface antigen T3/Leu 4 epsilon chain, T cell surface glycoprotein CD3, T cell surface glycoprotein CD3 delta chain, T cell surface glycoprotein CD3 epsilon chain, T cell surface glycoprotein CD3 gamma chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, delta subunit of T3, T-cell antigen receptor complex, epsilon subunit of T3, T-cell antigen receptor complex, gamma subunit of T3, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 delta chain, T-cell receptor T3 gamma chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface glycoprotein CD3 gamma chain, T3, T3d, T3e, T3g, T3z, TCRE, TCRk, Tcrz, TCRzeta) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of CD3D biology, including known isoforms, interactors, and disease-relevant expression patterns in immunology.

Common research applications

• WB: Western blot confirms target molecular weight and distinguishes isoforms under denaturing conditions. Expected band size should align with reported MW.
• ELISA: Sandwich or indirect ELISA can quantify soluble target in biological fluids or culture supernatants. Ensure samples fall within the linear detection range.

Notes for experimental interpretation

• Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
• Cross-reactivity: Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.
• Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.
• Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (11091247)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.