| Field | Specification |
|---|---|
| Target | |
| Alternative names | 4930549J05Rik; A430104F18Rik; AW552088; Cd247; CD247 antigen; CD247 antigen; zeta subunit; CD247 molecule; CD3; CD3 antigen; delta subunit; CD3 delta; CD3 epsilon; CD3 eta; CD3 gamma; CD3 molecule delta polypeptide; CD3 molecule; epsilon polypeptide; CD3 molecule; gamma polypeptide; CD3 zeta; CD3-DELTA; CD3d; CD3D antigen delta polypeptide; CD3d antigen; delta polypeptide (TiT3 complex); CD3d molecule delta; CD3d molecule delta CD3 TCR complex; CD3d molecule; delta (CD3-TCR complex); CD3D_HUMAN; CD3E; CD3e antigen; CD3E antigen epsilon polypeptide; CD3e antigen; epsilon polypeptide (TiT3 complex); CD3e molecule epsilon; CD3e molecule epsilon CD3 TCR complex; CD3e molecule; epsilon (CD3-TCR complex); CD3epsilon; CD3G; CD3g antigen; CD3G antigen gamma polypeptide; CD3g antigen; gamma polypeptide (TiT3 complex); CD3g molecule gamma; CD3g molecule gamma CD3 TCR complex; CD3g molecule; gamma (CD3-TCR complex); CD3H; CD3Q; CD3Z; CD3zeta; Ctg3; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD19; Leu-4; MGC138597; OKT3; delta chain; OTTHUMP00000032544; T cell receptor; T cell receptor T3 delta chain; T cell receptor T3 gamma chain; T cell receptor T3 zeta chain; T cell receptor zeta chain; T cell surface antigen T3/Leu 4 epsilon chain; T cell surface glycoprotein CD3; T cell surface glycoprotein CD3 delta chain; T cell surface glycoprotein CD3 epsilon chain; T cell surface glycoprotein CD3 gamma chain; T cell surface glycoprotein CD3 zeta chain; T-cell antigen receptor complex; delta subunit of T3; T-cell antigen receptor complex; epsilon subunit of T3; T-cell antigen receptor complex; gamma subunit of T3; T-cell antigen receptor complex; zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T3; T3d; T3e; T3g; T3z; TCRE; TCRk; Tcrz; TCRzeta |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Human CD3D |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-CD3D antibody raised in Rabbit, with confirmed utility in ELISA, WB, IF. It is designed to detect CD3D protein in Human, Mouse, Rat and supports researchers working in immunology contexts where consistent antibody performance is required.
Key elements and design rationale
- Immunogen: Human CD3D — the immunizing antigen determines the epitope region; confirm epitope compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents. Use matched secondaries to avoid no-signal or cross-reactivity issues.
- Polyclonal format: Recognizes multiple epitopes, providing robust signal across varied preparations and species variants. Inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Compatible with standard Protein A/G purification and widely supported by secondary reagents. Include an isotype-matched control at equivalent concentration.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes, reducing non-specific populations and improving signal-to-noise.
Biological background
CD3D (also referred to as 4930549J05Rik, A430104F18Rik, AW552088, Cd247, CD247 antigen, CD247 antigen, zeta subunit, CD247 molecule, CD3, CD3 antigen, delta subunit, CD3 delta, CD3 epsilon, CD3 eta, CD3 gamma, CD3 molecule delta polypeptide, CD3 molecule, epsilon polypeptide, CD3 molecule, gamma polypeptide, CD3 zeta, CD3-DELTA, CD3d, CD3D antigen delta polypeptide, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule delta, CD3d molecule delta CD3 TCR complex, CD3d molecule, delta (CD3-TCR complex), CD3D_HUMAN, CD3E, CD3e antigen, CD3E antigen epsilon polypeptide, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule epsilon, CD3e molecule epsilon CD3 TCR complex, CD3e molecule, epsilon (CD3-TCR complex), CD3epsilon, CD3G, CD3g antigen, CD3G antigen gamma polypeptide, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule gamma, CD3g molecule gamma CD3 TCR complex, CD3g molecule, gamma (CD3-TCR complex), CD3H, CD3Q, CD3Z, CD3zeta, Ctg3, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD19, Leu-4, MGC138597, OKT3, delta chain, OTTHUMP00000032544, T cell receptor, T cell receptor T3 delta chain, T cell receptor T3 gamma chain, T cell receptor T3 zeta chain, T cell receptor zeta chain, T cell surface antigen T3/Leu 4 epsilon chain, T cell surface glycoprotein CD3, T cell surface glycoprotein CD3 delta chain, T cell surface glycoprotein CD3 epsilon chain, T cell surface glycoprotein CD3 gamma chain, T cell surface glycoprotein CD3 zeta chain, T-cell antigen receptor complex, delta subunit of T3, T-cell antigen receptor complex, epsilon subunit of T3, T-cell antigen receptor complex, gamma subunit of T3, T-cell antigen receptor complex, zeta subunit of CD3, T-cell receptor T3 delta chain, T-cell receptor T3 gamma chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface glycoprotein CD3 gamma chain, T3, T3d, T3e, T3g, T3z, TCRE, TCRk, Tcrz, TCRzeta) is a protein target studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection and experimental design. Consult UniProt, NCBI Gene, and primary literature for current annotation of CD3D biology, including known isoforms, interactors, and disease-relevant expression patterns in immunology.
Common research applications
- ELISA: Sandwich or indirect ELISA can quantify soluble target in biological fluids or culture supernatants. Ensure samples fall within the linear detection range.
- WB: Western blot confirms target molecular weight and distinguishes isoforms under denaturing conditions. Expected band size should align with reported MW.
- IF: Immunofluorescence enables subcellular localization in fixed cells or tissue sections. Fixation and permeabilization conditions are critical for epitope accessibility.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins or isoforms. Orthogonal validation (siRNA, KO lysate, recombinant protein) is recommended to confirm signal specificity.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) can affect performance. Pilot dilution linearity and spike-recovery experiments are recommended for quantitative studies.
- Species reactivity: Confirmed for Human, Mouse, Rat. Extrapolation to untested species requires empirical validation given potential epitope sequence divergence.
This product is a polyclonal antibody purified from rabbit antiserum.
CD3D is a protein target in Human, Mouse, Rat biology. This polyclonal antibody raised in Rabbit is designed to detect CD3D in ELISA, WB, IF applications, with IgG isotype.
This antibody is reported reactive against Human, Mouse, Rat. The immunogen was derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
The supplier reports performance in ELISA, WB, IF. Each application requires independent dilution optimization, blocking conditions, and appropriate controls. Performance in unlisted applications requires empirical testing.
This is a non-conjugated IgG antibody requiring a compatible secondary for detection. Select an anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
Recommended: (1) Isotype control — IgG from Rabbit at matching concentration for non-specific background; (2) Positive control — known CD3D-expressing cell/tissue; (3) Negative control — knockdown or knockout sample to confirm signal specificity; (4) Dilution linearity — verify proportional signal decrease to confirm quantitative linearity in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
