| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Hygromycin, Puromycin, RFP (constitutively expressed), Zeocin |
| Shipping | |
| Species |
Background
CD40 is a cell surface receptor of the tumor necrosis factor receptor superfamily, expressed prominently on antigen-presenting cells such as B cells, macrophages, and dendritic cells. Engagement of CD40 by its ligand CD40L, expressed on activated T cells, delivers a critical co-stimulatory signal that licenses antigen-presenting cells, promotes B cell proliferation and antibody class switching, and enhances dendritic cell maturation. CD40 signaling proceeds through TRAF adaptor proteins to activate the transcription factor NF-κB, driving expression of genes that coordinate adaptive immune responses. CD40 is studied for its central role in T cell-dependent immunity and is an actively pursued target for cancer immunotherapy and vaccine development.
Product Description & Applications
The CD40/NF-κB Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human CD40 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable cell line that responds quantitatively to CD40 engagement with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media, allowing kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of antigen-presenting cell function, immune regulation, and CD40-targeting therapeutics in primary and difficult-to-transfect cells.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human CD40 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
