| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cd74; Ii; H-2 class II histocompatibility antigen gamma chain; Ia antigen-associated invariant chain; Ii; MHC class II-associated invariant chain; CD antigen CD74) [Cleaved into: Class-II-associated invariant chain peptide; CLIP)] |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Mouse H-2 class II histocompatibility antigen gamma chain protein (56-279AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-Cd74 antibody raised in Rabbit, conjugated with HRP, with confirmed utility in ELISA. Designed to detect Cd74 in Mouse, supporting researchers in immunology contexts.
Key elements and design rationale
- Immunogen: Recombinant Mouse H-2 class II histocompatibility antigen gamma chain protein (56-279AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
- HRP conjugate: Direct chromogenic (TMB/OPD) or chemiluminescent (ECL) detection. Quench endogenous peroxidase in tissue applications. No secondary required.
Biological background
Cd74 (also known as Cd74, Ii, H-2 class II histocompatibility antigen gamma chain, Ia antigen-associated invariant chain, Ii, MHC class II-associated invariant chain, CD antigen CD74) [Cleaved into: Class-II-associated invariant chain peptide, CLIP)]) is a protein studied in Mus musculus (Mouse) systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of Cd74 biology relevant to immunology.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Mouse; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
Cd74 is a protein target in Mouse biology. This polyclonal antibody raised in Rabbit detects Cd74 and is validated for ELISA applications.
This antibody is reported reactive against Mouse. Immunogen derived from Mus musculus (Mouse). Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
HRP enables direct chromogenic (TMB/OPD) or chemiluminescent (ECL) detection — no secondary needed. Block endogenous peroxidase in tissue applications before applying this antibody.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing Cd74; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
