| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Conjugate | |
| Host | |
| Immunogen | An EBV-transformed B cell line from a patient with MHC class II deficiency |
| Isotype | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Overview
CD74 FITC is a Mouse monoclonal targeting CD74, supplied as a FITC format for FC workflows. It supports measurement of Human target expression in common experimental systems.
Key elements and design rationale
- Clone: PIN.1 — consistent clone identity can support panel reproducibility and cross-study comparisons.
- Isotype: IgG1, k — informs selection of matched controls and secondary reagents when relevant.
- Conjugate: FITC — enables direct detection in fluorescence-based assays. Excitation is typically matched to Blue (488nm) lasers in cytometer configurations.
- Host species: Mouse — useful for panel design and control strategy planning.
- Reactivity: Human — interpret staining in the context of species-specific sequence and expression differences.
Key specifications such as clone identity, isotype, and fluorophore conjugation help researchers align panel design, control selection, and instrument configuration with the biological question and sample type.
Biological background
The clone PIN.1, a mouse monoclonal antibody, recognizes an ~33-35 kDa protein doublet corresponding to the apparent molecular mass of the p33 and p35 forms of the cytoplasmic tail of human CD74, a type II transmembrane glycoprotein. CD74 is known as an invariant chain or MHC class II chaperone or MIF (macrophage migration inhibitory factor) receptor. It plays a critical role in antigen processing, resulting in antigen presentation by MHC class II to CD4+ T cells. Antigen presenting cells such as B cells, monocytes/macrophages, dendritic cells, and Langerhans cells as well as activated T cells, carcinomas of lung, renal, gastric and thymic origins express CD74. CD44 is an integral member of the CD74 receptor complex and required for CD74 signal transduction. CD74’s cytoplasmic domain binds chromatin and regulates transcription and expression of genes involved in immune regulation, cell survival, and hematopoietic cancers. Published report claimed that the urease B subunit released by H. pylori binds to CD74 of gastric epithelial cells leading to induce NF-κB activation and IL-8 production.
Research relevance and current trends
- High-parameter immunophenotyping: combining CD74 with complementary lineage and activation markers to resolve complex cell states.
- Panel standardization and data comparability: increasing emphasis on consistent reagents, compensation-aware fluorophore choices, and shared gating strategies.
- Integration with single-cell multi-omics: pairing surface marker profiling with transcriptomic or proteomic readouts to connect phenotype to function.
Common research applications
- Flow cytometry: quantify CD74-positive populations and compare expression distributions across conditions or time points.
- Cell sorting: enrich CD74-defined subsets for downstream RNA/protein assays or functional readouts.
Changes in measured signal are typically interpreted in the context of cell subset frequency, activation/differentiation state, and sample processing effects rather than as a standalone readout.
Notes for experimental interpretation
- Fluorophore selection: consider brightness, spectral overlap, and instrument configuration; compensation and spillover can affect apparent population boundaries.
- Biology-driven confounders: activation state, differentiation, and isoform/PTM variation can shift epitope accessibility and apparent expression.
- Control concepts: include matched isotype and fluorescence-minus-one (FMO) controls where appropriate, and interpret results alongside biological positive/negative reference samples.
For antibody-based assays, monoclonal versus polyclonal format can influence epitope recognition breadth and signal consistency. Conjugated antibodies support direct detection and can simplify multicolor panel design when paired with appropriate controls and instrument settings.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
