| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | T-Cell Surface Glycoprotein CD8, T-Cell Surface Antigen T8, CD8 Molecule |
| Product Type | |
| Shipping | |
| Species | |
| Storage |
Product Description
Immune Cell MarkersCryopreserved vial (10 x 10^6 cells) of CD8+ T cells that were negatively selected from freshly isolated primary human peripheral blood mononuclear cells (PBMCs). The PBMCs came from a healthy donor, and were isolated from whole blood or leukapheresis samples using a Ficoll gradient. Magnetic antibodies to monocytes, granulocytes, CD4+ T cells, gamma/delta T cells and other immune subsets present in PBMCs were then used to purify untouched CD8+ T cells via immunomagnetic separation. Before and after CD8+ T cell isolation, the cells were stained to evaluate purity and viability by flow cytometry. Cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stemcell, #07930) at a controlled rate. Source
Normal human PBMC from Leukapheresis Sample
Product Specifications
| Harmonized Tariff Code | 3002-5900 |
|---|
Usage Notes
Handling Directions
First, pre-warm Immuno Culture Medium to 37°C. It is important to work quickly in the following
steps to ensure high cell viability and recovery. Quickly thaw cells in a 37°C water bath with
constant but gentle agitation. Clean the outside of the vial with 70% ethanol and immediately
transfer the entire contents to a 50 ml tube. Slowly add 10 ml of pre-warmed medium while gently
swirling the tube to mix. Centrifuge the cell suspension at 300 x g for 15 minutes at room
temperature. Carefully remove the supernatant with a pipette without disturbing the pellet. Gently
resuspend the cell pellet by flicking the tube, then add the desired volume of warm medium, and
mix. NOTE: Up to 30% cell loss can be expected during washing steps. Cells are now ready for
use in downstream applications.
Regulatory Information
License Disclosure
Cell count, viability (trypan blue exclusion and FACS with impermeable DNA binding dye), and surface expression of CD3 and CD4
Related Products
Related Products: Cat. #90184-B, 90184-A, 79704, 79752
This product is classified as BSL-1. Standard microbiological practices (gloves, lab coat, eye protection) are sufficient. No specialized containment facility is required beyond a clean bench. Consult your institutional IBC for GMO registration requirements.
We recommend performing mycoplasma testing upon receipt and at regular intervals during culture, regardless of vendor QC status. Validated detection methods include PCR-based assays (e.g., Venor™GeM) and luminescence assays (e.g., MycoAlert™). Contamination can be introduced during routine handling.
Store this product at Store cells at -135°C or colder. Thawed . Specifically: Store cells at -135°C or colder. Thawed cells should be used immediately for downstream applications. Because these are primary cells, we do not recommend maintaining these cells in culture for long periods of time. Transfer cells from dry-ice shipping to the recommended storage immediately upon receipt. Avoid repeated freeze-thaw cycles, which reduce viability and may alter expression characteristics.
A standard research-use license applies (No). Commercial applications or transfer to other facilities may require a separate license. Contact BPS Bioscience for details.
Can't find the cell line you need—or require a custom engineered model for your study? We offer end-to-end support for diverse research needs, including:
- Cell line sourcing and selection (species, tissue, and disease model matching)
- Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA)
- Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs)
- Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles)
- Inducible expression systems (Tet-On/Off and regulatable constructs)
- Drug resistance marker selection (puromycin, G418, hygromycin, and others)
- Custom growth and media optimisation for specific assay requirements
- Scale-up production for high-throughput screening campaigns
- Authentication and QC services (STR profiling, mycoplasma testing, viability assessment)
Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
