| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 4F9; Antigen detected by monoclonal and IA4; C33; C33 antigen; CD 82; CD82; CD82 antigen; CD82 molecule; CD82_HUMAN; GR 15; GR15; IA 4; IA4; Inducible membrane protein; Inducible membrane protein R2; KAI 1; KAI1; Kangai 1 (suppression of tumorigenicity 6; prostate; CD82 antigen (R2 leukocyte antigen; antigen detected by monoclonal and IA4)); Kangai 1; Kangai1; Leukocyte surface antigen R2; Metastasis suppressor Kangai 1; Metastasis suppressor Kangai-1; Metastasis suppressor Kangai1; Prostate cancer antimetastasis gene KAI1; R2; R2 leukocyte antigen; SAR 2; SAR2; ST 6; ST6; Suppression of tumorigenicity 6; Suppression of tumorigenicity 6 prostate; Suppressor of tumorigenicity 6; Suppressor of tumorigenicity 6 protein; Tetraspanin 27; Tetraspanin-27; Tetraspanin27; Tspan 27; Tspan-27; Tspan27 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human CD82 antigen protein (111-228AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-CD82 antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect CD82 in Human for immunology research.
Key elements and design rationale
- Immunogen: Recombinant Human CD82 antigen protein (111-228AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- Biotin: Streptavidin/avidin amplification; block endogenous biotin in biotin-rich samples.
Biological background
CD82 (also: 4F9, Antigen detected by monoclonal and IA4, C33, C33 antigen, CD 82, CD82, CD82 antigen, CD82 molecule, CD82_HUMAN, GR 15, GR15, IA 4, IA4, Inducible membrane protein, Inducible membrane protein R2, KAI 1, KAI1, Kangai 1 (suppression of tumorigenicity 6, prostate, CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and IA4)), Kangai 1, Kangai1, Leukocyte surface antigen R2, Metastasis suppressor Kangai 1, Metastasis suppressor Kangai-1, Metastasis suppressor Kangai1, Prostate cancer antimetastasis gene KAI1, R2, R2 leukocyte antigen, SAR 2, SAR2, ST 6, ST6, Suppression of tumorigenicity 6, Suppression of tumorigenicity 6 prostate, Suppressor of tumorigenicity 6, Suppressor of tumorigenicity 6 protein, Tetraspanin 27, Tetraspanin-27, Tetraspanin27, Tspan 27, Tspan-27, Tspan27) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current CD82 annotation relevant to immunology.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
CD82 is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects CD82 in ELISA applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Biotin: streptavidin/avidin amplification. Block endogenous biotin in biotin-rich samples.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing CD82; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
