CDKN1A Antibody / p21

Overview

CDKN1A antibody supplied as a purified reagent for IF, WB, IHC-P in Human, Mouse samples. This product is a polyclonal (rabbit origin) antibody (host: Rabbit; isotype: Rabbit Ig) intended for research use only. The target is commonly annotated with nuclear localization context, which may inform staining patterns.

Key elements and design rationale

• Antibody identity: Polyclonal (rabbit origin); host Rabbit; isotype Rabbit Ig.
• Format and purification: format: Purified; purity: Antigen affinity purified.
• Species reactivity (reported): Human, Mouse.
• Applications (listed): IF, WB, IHC-P.
• Immunogen / epitope context: A portion of amino acids 133-164 from the human protein was used as the immunogen for the CDKN1A antibody..
• Localization: Nuclear (annotation-level guidance; cell state and isoforms can shift patterns).

These attributes help you align the antibody with the biological question (target state, sample type, and readout) while keeping interpretation grounded in appropriate controls.

Biological background

CDKN1A is the intended antigen for this primary antibody. Reported biological context includes: May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin- dependent kinase substrates and blocking cell cycle progression. Subcellular localization information (Nuclear) can be useful when interpreting IF/ICC patterns and selecting compartment-enriched lysates for WB.

Research relevance and current trends

• Post-translational modification mapping: phosphorylation-site–resolved antibodies are used to connect signaling inputs to target activation states and downstream readouts.
• Signal-flow and turnover studies: researchers pair immunodetection with perturbations that modulate enzymatic activity or proteostasis to understand regulation, stability, and feedback.
• Spatial and single-cell approaches: imaging-based and cytometry workflows increasingly quantify heterogeneity and relocalization rather than only bulk abundance.

Common research applications

• Immunofluorescence (IF): visualize localization and co-localization patterns in cells or tissues.
• Western blot (WB): compare relative abundance/isoform patterns across conditions and sample types; band shifts may reflect processing or post-translational modification.
• IHC-P: commonly used to measure relative target levels or localization changes in the context of the experimental question.

Across these readouts, differences in signal intensity, localization, or complex enrichment are typically interpreted alongside sample-matched controls and independent evidence to distinguish regulation from technical variation.

Notes for experimental interpretation

• Isoforms, cleavage products, or post-translational modifications can alter apparent molecular weight and subcellular distribution; interpret bands and staining patterns in the context of expected biology and sample preparation.
• Species differences and epitope conservation may affect binding; use matched positive controls and orthogonal evidence when comparing across organisms.
• Control concepts: include appropriate isotype and secondary-only controls (for imaging), and consider genetic perturbations (knockout/knockdown/overexpression) or independent antibodies targeting distinct epitopes to strengthen conclusions.

Epitope context is defined by the immunogen description; when available, align this with known domains, PTM sites, or family homology to anticipate potential cross-reactivity patterns. As a polyclonal antibody, recognition spans multiple epitopes, which can improve detection across conformations but may broaden background depending on sample context.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.