| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Biological Activity | |
| Enzyme Type | |
| Expression System | |
| Form | Liquid |
| Formulation | |
| Product Type | |
| Purity | |
| Source | Sf9, Baculovirus cells. |
| Species | |
| Storage | |
| Target |
Product Description
Carboxylesterase 2E Mouse Recombinant
Overview
Carboxyl ester lipase also known as CEL, formely called cholesterol esterase or bile salt-stimulated lipase, is an enzyme with lipolytic capablity of hydrolyzing cholesteryl esters, tri-, di-, and mono- phospholipids, acylglycerol, ceramide and lysophospholipids. The carboxyl terminus of the enzyme controls enzymatic activity by creating hydrogen bonds with the surface loop to partlyshield the active site. The active catalytic site triad of serine-histidine-aspartate is centrally located in the enzyme structure and is partly covered by a surface loop. Bile salt binding to the loop domain set free the active site for accessibility by water-insoluble substrates. CEL is produced mainly in the pancreas and lactating mammary gland, thus the protein is also expressed in liver, macrophages, and in the vessel wall.
Biological Activity
Specific activity is > 100,000 pmol/min/ug. Measured by the amount of enzyme that hydrolyze 1.0 umole of p-nitrophenyl butyrate to p-nitrophenol per minute at pH7.5 at 25C˚.
Specifications
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
|---|---|
| Formulation | The CEL solution (0.5 mg/ml) contains 10% Glycerol and Phosphate-Buffered Saline (pH 7.4). |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Expression System | Sf9, Baculovirus cells. |
