| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | CFTR, cystic fibrosis transmembrane conductance regulator |
| Biological Activity | |
| Product Type | |
| Shipping | |
| Species | |
| Storage |
Scientific Background
Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is encoded by the CFTR gene. CFTR is an ABC transporter-class ion channel that transports chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affect functioning of the chloride ion channels in these cell membranes, leading to cystic fibrosis. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.
Product Description
ion channelThe CFTR HEK293 Cell Line expresses full length, wild-type human cystic fibrosis transmembrane conductance regulator (CFTR) protein (Genbank #P13569, NP_000483.3), with a C-terminal Streptavidin-Binding Peptide (SBP) tag. Expression must be induced ≥24 hours prior to an experiment using 1 µg/ml Doxycycline and 3 mM Na-butyrate. The inducible expression of CFTR was confirmed by Western blotting and flow cytometry.
Product Specifications
| Host Cell Line | HEK293 |
|---|---|
| Host Species | Human |
| Transfection Method | Lipofectamine 2000 |
| Supplied As | Each vial contains ~2 X 10^6 cells in 1 ml of Sigma Freezing Medium (Cat. # C-6164). |
| Harmonized Tariff Code | 3002-5900 |
Usage Notes
See cell line data sheet for detailed culturing and assay protocol.
Safety & Handling
⚠ Avoid freeze/thaw cycles.
Regulatory Information
License Disclosure
Related Products
Related Products: Cat. #60181, 79535, 79631, 78345
Required Accessories: Cat. #60181,79535,79631
This product is engineered on a HEK293 background (Human origin). The HEK293 host was selected for its compatibility with stable transfection and the target pathway or assay type. Consult the product datasheet for passage number guidance and recommended culture media.
This product is classified as BSL-2. Work must be performed in a certified BSL-2 laboratory by trained personnel. All procedures that may generate aerosols must be conducted inside a Class II Biological Safety Cabinet. Institutional Biosafety Committee (IBC) approval is required before use.
We recommend performing mycoplasma testing upon receipt and at regular intervals during culture, regardless of vendor QC status. Validated detection methods include PCR-based assays (e.g., Venor™GeM) and luminescence assays (e.g., MycoAlert™). Contamination can be introduced during routine handling.
Store this product at Liquid Nitrogen. Specifically: Store in liquid nitrogen immediately upon receipt. Cells are stable after a minimum of seven continuous passages. Upon first use, amplify the cells in culture and make several frozen aliquots for future use. Transfer cells from dry-ice shipping to the recommended storage immediately upon receipt. Avoid repeated freeze-thaw cycles, which reduce viability and may alter expression characteristics.
Yes, a license is required (Yes). Purchase of this cell line grants a time-limited research-use license for use in your immediate laboratory only. This license does not permit redistribution, sub-licensing, transfer to other institutions, or commercial use. Refer to the License Disclosure section on this page or contact BPS Bioscience for details regarding modifications or commercial licensing.
This stable cell line was generated using Lipofectamine 2000 for transgene delivery into the parental host. The stably integrated cells were selected using the appropriate resistance marker and verified for expression prior to cryopreservation.
Can't find the cell line you need—or require a custom engineered model for your study? We offer end-to-end support for diverse research needs, including:
- Cell line sourcing and selection (species, tissue, and disease model matching)
- Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA)
- Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs)
- Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles)
- Inducible expression systems (Tet-On/Off and regulatable constructs)
- Drug resistance marker selection (puromycin, G418, hygromycin, and others)
- Custom growth and media optimisation for specific assay requirements
- Scale-up production for high-throughput screening campaigns
- Authentication and QC services (STR profiling, mycoplasma testing, viability assessment)
Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
- Gadsby, D.C., et al. Nature 440 (7083): 477–483 (2006).
- Hillier, L.W., et al. Nature 424:157-164 (2003).
- McCann, C. M., et al. BioTechniques 38 (6):945–952 (2005).
