| Field | Specification |
|---|---|
| Mfr No | |
| Species |
The Chang Liver cell line, originally believed to be derived from normal human liver tissue, has undergone significant reclassification following advanced genetic profiling. STR PCR DNA profiling techniques have demonstrated that the Chang Liver cell line is indistinguishable from the HeLa cell line, suggesting that it is not derived from hepatocyte cells as previously thought, but rather should be considered a HeLa derivative. This revelation has important implications for researchers using this cell line, emphasizing the need for careful interpretation of experimental results derived from its use.
HeLa cells, originally taken from Henrietta Lacks, a Black woman, in the early 1950s, are known for their robust growth and genetic stability in vitro, characteristics likely shared by the Chang Liver cell line given its genetic similarity. This background necessitates that studies employing the Chang Liver cell line in research related to liver function or diseases may need to be re-evaluated or confirmed with additional hepatocyte-specific models. The misidentification also highlights broader issues in cell culture practices, including cross-contamination and mislabeling, underscoring the importance of regular authentication of cell lines used in research settings.
—
- Isoenzymes: G6PD, A
- Tumorigenic: Yes, in Syrian hamsters
- Viruses: Tested MHV (mouse hepatitis virus) negative
- Virus susceptibility: Poliovirus 1, 2, 3, adenovirus 3, vesicular stomatitis (Indiana)
- Reverse transcriptase: negative
- Products: keratin
- cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
- supplements: Supplement the medium with 10% FBS and 1% NEAA
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 x 104 cells/cm2 will yield in a confluent layer in about 4 days
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
10%
OFF
15%OFF
20%
OFF
$50
OFF
FREE
SAMPLE
