| Field | Specification |
|---|---|
| Applications | |
| Assay type | |
| Sample type(s) | Serum | Plasma |
| Detection method | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
The Chicken Mycoplasma Gallisepticum Antibody ELISA Kit detects antibodies against Chicken Mycoplasma Gallisepticum in serum and plasma. The 96-well microplate is pre-coated, and the colorimetric signal is read at 450 nm.
Specifications
| Assay Type | Qualitative |
|---|---|
| Detection Method | Colorimetric (OD 450 nm) |
| Sample Type | Serum, Plasma |
| Kit Size | 96 T |
| Shipping | 2-8 °C |
Kit Components
| Component | Quantity |
|---|---|
| ELISA Microtiter plate | 96 wells |
| Dilution Plate | 96 wells |
| HRP Conjugate | 12 mL |
| 10×Concentrated Wash Buffer | 50 mL |
| Substrate Reagent A | 6 mL |
| Substrate Reagent B | 6 mL |
| Sample Diluent | 50 mL |
| Stop Solution | 6 mL |
| Positive Control | 0.8 mL |
| Negative Control | 0.8 mL |
| Plate Sealer | 3 pieces |
| Sealed Bag | 1 piece |
| Manual | 1 copy |
Assay Procedure
Bring all reagents and samples to room temperature (25 °C) before use. All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2-8 °C.
- Number the sample and control in order (in duplicate), and keep a record of control wells and sample wells. Set 1 well for blank/negative/positive control. Run samples in duplicate.
- Add 100 µL of positive/negative control to positive/negative control well, add 100 µL of Sample Diluent to blank control well and add 100 µL of Diluted serum to the sample wells.
- Cover with a plate sealer and mix thoroughly, incubate at 37 °C for 30 min protected from light.
- Remove the liquid in each well. Immediately add 300 µL of Wash Buffer to each well and wash. Repeat 3 times, soaking 1 min each time. Invert the plate and pat it against thick clean absorbent paper (If bubbles exist in the wells, clean tips can be used to prick them).
- Add 100 µL of HRP Conjugate to each well, cover with a plate sealer and incubate at 37 °C for 30 min protected from light.
- Repeat step 4 for washing.
- Add 50 µL of Substrate Reagent A and 50 µL of Substrate Reagent B to each well. Cover with a plate sealer and mix thoroughly, incubate at 37 °C for 10 min protected from light.
- Add 50 µL of Stop Solution to each well, mix thoroughly.
- Measure the absorbance of each well by using a microplate reader with 450 nm/630 nm wavelength. This step should be finished in 10 min after stop reaction.
Required but not supplied: a microplate reader for 450 nm (dual wavelength 450 nm / 630 nm recommended), precision pipettes and tips, a 37 °C incubator or water bath, deionized or distilled water, and absorbent paper.
Result Interpretation
- Cut Off (C.O) =0.25+Average of negative control ODs (when Average of negative control ODs < 0.05, it is calculated as 0.05.)
- Positive result: Sample OD > Cut Off (C.O)
- Negative result: Sample OD < Cut Off (C.O)
Storage & Handling
For Research Use Only. Not for use in diagnostic procedures.
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