| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Alternative Names | CHIR99021 |
| Cas No. | |
| Molecular Weight | |
| Product Type | |
| Signaling Pathway | |
| SMILES | |
| Target |
Compound Overview
CHIR-99021 (CAS 252917-06-9) is a research-grade small molecule supplied by TargetMol.
Alternative names: CHIR99021
Physical Properties
| CAS Number | 252917-06-9 |
|---|---|
| Molecular Formula | C22H18Cl2N8 |
| Molecular Weight | 465.34 g/mol |
| SMILES | ClC1=C(C=2C(=CN=C(NCCNC3=CC=C(CN)C=N3)N2)C=4NC(C)=CN4)C=CC(Cl)=C1 |
| Form | Solid |
| Shipping | Room Temperature (RT) |
Biological Activity
CHIR-99021 (CT99021) is an activator of the Wnt/beta-catenin signaling pathway and a GSK-3alpha/beta inhibitor (IC50=10/6.7 nM) with selective and oral activity.CHIR-99021 induces cellular autophagy, which enhances self-renewal in mouse and human embryonic stem cells.
Target(s): GSK-3, Wnt/beta-catenin, Autophagy
Signaling pathway(s): Cytoskeletal Signaling, Autophagy, PI3K/Akt/mTOR signaling, Stem Cells
Safety & Regulatory
For Research Use Only (RUO). Not for therapeutic, diagnostic, or clinical use. Wear appropriate PPE and consult the Safety Data Sheet (SDS) prior to handling.
When solubility is listed as < 1 mg/mL, the compound dissolves to less than 1 mg per milliliter in water at 25 °C, pH 7.4. Such compounds require an organic co-solvent such as DMSO to prepare a concentrated stock, which is then diluted into aqueous assay buffer. For cell-based assays, keep the final DMSO concentration below 0.1% (v/v) to avoid solvent-induced cytotoxicity.
It is not recommended to store DMSO stock solutions at room temperature. Once the stock solution is prepared, aliquot into single-use volumes and store at −20 °C (or −80 °C for sensitive compounds). Room-temperature storage accelerates chemical degradation and moisture uptake. When ready to use, thaw an aliquot at room temperature, use immediately, and avoid repeated freeze-thaw cycles.
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
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