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Disclaimer: The prices displayed for cell lines are exclusively for not-for-profit customers. If you represent a commercial entity, please contact us for alternative pricing.The CHO-CXCR4-Medium-high cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line expressing the CXCR4 receptor at a medium-high level, approximately 9500 molecules per cell. This cell line was developed using an innovative landing pad technology, which ensures targeted integration of the CXCR4 gene at a pre-validated genomic locus. This approach results in consistent and reliable expression of the CXCR4 receptor, facilitating reproducible experimental outcomes.
CXCR4, also known as CD184, is a chemokine receptor involved in critical biological processes such as immune cell trafficking, hematopoiesis, and as a co-receptor for HIV entry into cells. The receptor's interaction with its ligand, CXCL12, is essential for the migration and homing of hematopoietic stem cells and leukocytes. In oncology, CXCR4 plays a significant role in tumor growth, metastasis, and angiogenesis, with its expression often upregulated in various cancers, including hematological malignancies. This upregulation is frequently associated with therapy resistance and poor prognosis. The expression of CXCR7 in this cell line was confirmed using flow cytometry.
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Receptors expressed: CXCR4 (CD184)
- cultureMedium: For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039)
- supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL.
- dissociationReagent: For adherent cultures: Trypsin-EDTA
- subculturing: For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C for 5-10 minutes, or until the cells detach. Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO2, and change the medium every 2-3 days.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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