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Disclaimer: The prices displayed for cell lines are exclusively for not-for-profit customers. If you represent a commercial entity, please contact us for alternative pricing.The CHO-CXCR7-Medium-high cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line engineered to express the CXCR7 receptor at a medium-high level. This cell line was created using an innovative landing pad technology, which allows for targeted integration of the CXCR7 gene at a pre-validated genomic locus, ensuring consistent and reproducible expression. CXCR7, also known as ACKR3, is an atypical chemokine receptor involved in immune modulation and cancer biology. Unlike typical GPCRs, CXCR7 does not signal through G proteins but instead scavenges chemokines such as CXCL12 and CXCL11, and forms heterodimers with CXCR4, influencing processes like tumor progression, metastasis, and angiogenesis.
CXCR7 is notably overexpressed in various cancers, including breast, lung, and prostate cancers, where it is linked to increased tumor growth, metastasis, and poorer prognosis. This makes the CHO-CXCR7-Medium-high cell line particularly valuable for oncology research, allowing for the study of CXCR7's role in cancer progression and its potential as a therapeutic target. The expression of CXCR7 in this cell line was confirmed using flow cytometry.
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Receptors expressed: CXCR7 (ACKR3)
- cultureMedium: For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039)
- supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL.
- dissociationReagent: For adherent cultures: Trypsin-EDTA
- subculturing: For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C for 5-10 minutes, or until the cells detach. Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO2, and change the medium every 2-3 days.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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