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| Product format | Frozen |
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Overview
CHO-F is a suspension-adapted Chinese Hamster Ovary (CHO) cell line developed from CHO-ES for high-efficiency recombinant protein expression. CHO-F cells demonstrate robust growth in serum-free suspension culture and are suitable for both transient and stable expression of recombinant antibodies and proteins.
Key elements and design rationale
- Model identity: CHO-F is supplied as an immortalized cell line derived from Chinese Hamster ovary.
- Growth properties: Suspension, round
- Growth conditions: Serum-Free CHO Media (TM098) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Product format: Frozen, BSL-2
This cell-based model is generally used in ovary biology, phenotype comparison, and assay development studies. Donor/background information is available for contextual interpretation.
Biological background
The cell line is readily scalable from small-volume shake flasks to bioreactor systems while maintaining consistent growth and viability. For optimal transfection efficiency in CHO-F cells, Susfectin™ Transfection Reagent (G4000) is recommended. Susfectin™ has been validated for use with suspension CHO cells and supports high expression levels in transfection workflows. Key FeaturesSuspension-adapted CHO cellsSerum-free growth capabilityHigh transfection efficiencySuitable for recombinant antibody and protein expressionScalable from shake flasks to bioreactorsConsistent growth performance and high viability Donor/background information provided for this product: Female, Adult, Chinese hamster.
Research relevance and current trends
- Cultured cell-line models remain central to in vitro studies of phenotype, signaling, and pathway regulation under controlled conditions.
- Researchers commonly compare morphology, growth rate, and marker expression across media formulations, treatments, or time courses.
- Interpretation is generally strengthened by using matched controls, consistent passage handling, and appropriate culture surfaces.
Common research applications
- Routine expansion and maintenance of a defined cell model for downstream in vitro experiments.
- Phenotype, signaling, or marker-expression studies performed under standardized culture conditions.
- Cell-based assay development in which passage number, growth surface, and medium composition are tracked as experimental variables.
Changes in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.
Notes for experimental interpretation
- Morphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.
- Matched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.
Culture and product details
- Growth Conditions: Serum-Free CHO Media (TM098) + 1% Penicillin/Streptomycin Solution (G255), 37.0°C, 5% CO₂.
- Warranty: abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period”.
- Thaw cells quickly in a 37°C water bath while agitating gently (maximum 2 minutes). The vial cap should be kept above the water level to minimize the risk of contamination.
- Decontaminate the vial by spraying and wiping the exterior of the vial with 70% ethanol. From this point onwards, all operations should be strictly carried out inside a biological safety cabinet using aseptic conditions.
- Transfer the cell suspension into a 15ml sterile conical tube containing 5ml of pre-warmed, complete growth media. Centrifuge cells at 125xg for 5-7 minutes.
- Aspirate the supernatant without disturbing the cell pellet. Re-suspend the cell pellet in fresh pre-warmed, complete growth media and seed at 2–3 × 10⁵ cells/ml in a shake flask.
- Place flask on an orbital shaker in a humidified incubator.
- Monitor cell density and viability daily. Passage cells when density reaches 3–5 × 10⁶ cells/ml.
- Dilute cells to 2–3 × 10⁵ cells/ml with fresh medium.
- Alternatively, replace complete growth media by centrifugation and re-suspend the cell pellet in fresh complete media, and add appropriate aliquots of the cell suspension to new culture vessels, as desired. Recommend 2–3 × 10⁵ cells/ml in a shake flask.
- Incubate the cells at the recommended conditions. Maintain cells in exponential growth phase for best performance.
How should I handle live cells once I receive them?
https://www.abmgood.com/immortalized-cells-documents.html
Following these guidelines will help ensure optimal cell viability and performance.
Why are these cells classified as biosafety level II?
What is your warranty or return policy?
Please refer to the following link for full information:
https://www.abmgood.com/terms
For additional questions, our Order team is happy to assist and can be reached at order@abmgood.com.
How many times can cells divide?
Primary cells have a limited lifespan and will undergo a finite number of population doublings before entering senescence. The exact number varies by cell type and culture conditions.
Immortalized cell lines are capable of extended or indefinite proliferation under proper culture conditions, although growth characteristics may vary between lines.
Do I need Applied Cell Extracellular Matrix (G422) if I am using PriCoat™ flasks?
Cell line sourcing and selection (species, tissue, and disease model matching) · Stable cell line engineering (overexpression, knockdown, knockout via CRISPR/Cas9, shRNA, sgRNA) · Reporter gene integration (GFP, RFP, luciferase, fluorescent/bioluminescent constructs) · Genome editing and knockin (point mutations, tagged endogenous proteins, conditional alleles) · Inducible expression systems (Tet-On/Off and regulatable constructs) · Drug resistance marker selection (puromycin, G418, hygromycin, and others) · Custom growth and media optimisation for specific assay requirements · Scale-up production for high-throughput screening campaigns · Authentication and QC services (STR profiling, mycoplasma testing, viability assessment). Talk to a Scientist or contact support@biohippo.com.
