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Disclaimer: The prices displayed for cell lines are exclusively for not-for-profit customers. If you represent a commercial entity, please contact us for alternative pricing.The CHO-FOLR1 cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line engineered to express the FOLR1 receptor at a medium-high level, approximately 15,000 molecules per cell. This cell line was developed using advanced landing pad technology, which ensures precise and reproducible integration of the FOLR1 gene at a specific, pre-validated genomic locus. FOLR1, also known as Folate Receptor Alpha (FRα) or FBP, is a GPI-anchored membrane protein with high affinity for folate, facilitating its transport into cells. FOLR1 is significantly overexpressed in various epithelial cancers, including ovarian, breast, and non-small cell lung cancers, making it a valuable target for cancer immunotherapies, including CAR T cell therapies and bispecific antibodies.
The expression of FOLR1 in this cell line was confirmed using flow cytometry with a target-specific antibody, ensuring reliable and consistent receptor density across the cell population.
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Receptors expressed: FOLR1 (Folate Receptor Alpha (FRα) or FBP)
- cultureMedium: For adherent cultures: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)For suspension cultures: CHO Growth Medium A (from InSCREENeX; InSCREENeX catalog number INS-ME-1039)
- supplements: For adherent cultures: Supplement the medium with 5% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL.
- dissociationReagent: For adherent cultures: Trypsin-EDTA
- subculturing: For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C for 5-10 minutes, or until the cells detach. Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO2, and change the medium every 2-3 days.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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