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Disclaimer: The prices displayed for cell lines are exclusively for not-for-profit customers. If you represent a commercial entity, please contact us for alternative pricing.The CHO-HER2-Medium-high cell line is a stable recombinant CHO (Chinese Hamster Ovary) cell line that expresses the HER2 receptor at a medium-high level, approximately 13,000 molecules per cell. This cell line was developed using an innovative landing pad technology, ensuring the HER2 gene's targeted integration at a specific, pre-validated genomic locus. HER2, also known as ERBB2 or CD340, is a member of the epidermal growth factor receptor (EGFR) family and plays a critical role in regulating cell growth and differentiation. It is a significant biomarker and target in breast and ovarian cancers, where its overexpression is associated with increased tumor aggressiveness and poorer patient outcomes. The expression of CXCR7 in this cell line was confirmed using flow cytometry.
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Receptors expressed: HER2
- cultureMedium: Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
- supplements: Supplement the medium with 10% FBS. Add Geneticin (G418-Sulfat) to achieve a final concentration of 0.5 mg/mL.
- dissociationReagent: Trypsin-EDTA
- subculturing: For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C until the cells detach (5-10 minutes). Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO2, and change the medium every 2-3 days.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, split the cells at a ratio of 1:2 to 1:3 in T25 flasks and allow the cells to recover from the freezing process and to adhere (for adherent cultures) for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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