| Field | Specification |
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| Detection Range | |
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| Target |
Assay Principle
Tribioscience’s CHO Host Cell Protein ELISA is designed to quantitatively detect CHO HCP levels in samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 1 to 810 ng/mL. The levels of HCP samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for CHO protein.
Typical Data This standard curve (R 2 =0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity The minimum detectable dose (MDD) of CHO HCP is typically 1 ng/mL.
CHO Host Cell Protein ELISA Kit is a Solid-phase sandwich ELISA for the quantitative determination of CHO Host Cell Protein. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | CHO Host Cell Protein |
|---|---|
| Assay Type | Sandwich |
| Test Principle | Solid-phase sandwich ELISA |
| Sensitivity | 1 ng/mL |
| Detection Range | 1–810 ng/mL |
| Precision | Intra-assay CV 6.6%; Inter-assay CV 9% |
| Standard | Recombinant CHO Host Cell Protein |
| Specificity | This assay recognizes natural and recombinant CHO HCP. |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This assay has a sensitivity of 1 ng/mL and a detection range of 1–810 ng/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
