| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Promoter | |
| Reporter | |
| Selection Marker | Blasticidin, Puromycin |
| Shipping | |
| Species |
Background
The carbohydrate response element (ChoRE) is a DNA motif that mediates transcriptional responses to glucose and dietary carbohydrate. It is bound by ChREBP (carbohydrate response element-binding protein, encoded by MLXIPL) in partnership with its dimerization cofactor MLX. In response to elevated intracellular glucose metabolites, ChREBP translocates to the nucleus and binds ChoREs to activate genes involved in glycolysis and de novo lipogenesis, coordinating the conversion of excess carbohydrate into lipid. ChREBP is a key regulator of hepatic and adipose metabolism, and its activity is implicated in obesity, insulin resistance, fatty liver disease, and type 2 diabetes, making ChoRE-driven reporters valuable tools in metabolic research.
Product Description & Applications
The ChoRE Reporter Lentivirus places a reporter gene under the control of tandem carbohydrate response elements, providing a sensitive readout of ChREBP/MLXIPL transcriptional activity in human and mouse cells. Reporter options include fluorescent (GFP, RFP, EGFP, mCherry) and luminescent (firefly and Renilla luciferase) formats, with blasticidin or puromycin selection for stable reporter cell line establishment. Signal can be measured by fluorescence microscopy, flow cytometry, or luminometry.
The particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and cryopreserved cultures. Applications include monitoring glucose-responsive transcription, studying carbohydrate and lipid metabolism, and screening compounds that modulate ChREBP activity.
About This Product
This reporter lentivirus places a EGFP, Firefly Luc, mCherry, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Carbohydrate metabolism (ChREBP/MLXIPL) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
