| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human NARFL/CIAO3 recombinant protein (Position: Y196-W476) was used as the immunogen for the CIAO3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CIAO3 Antibody / Cytosolic iron-sulfur assembly component 3 is a anti-CIAO3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: CIAO3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
CIAO3 is encoded by the CIAO3 gene located on human chromosome 14q32.31. The protein is approximately 28 kilodaltons in size and belongs to the NUBP (nucleotide-binding protein) family of P-loop NTPases. It interacts with other CIA machinery components, including MMS19, CIAO1, and FAM96B (CIAO2B), forming a functional complex responsible for Fe-S cluster transfer to client proteins. CIAO3 contains conserved Walker A and Walker B motifs, enabling nucleotide binding and hydrolysis that regulate its conformational dynamics during cofactor delivery.
The CIAO3 antibody detects a 53 kilodalton band by western blot.rafficking. Functional studies show that depletion of CIAO3 results in defective maturation of cytosolic aconitase, DNA polymerases, and helicases, leading to impaired DNA replication and increased oxidative stress sensitivity. CIAO3-deficient cells exhibit reduced activity of Fe-S-dependent enzymes and compromised genome integrity.
CIAO3 also participates in iron homeostasis and redox regulation by influencing intracellular iron distribution. Disruption of the CIA machinery contributes to mitochondrial dysfunction, neurodegeneration, and metabolic disorders. Given its conserved function across species, CIAO3 serves as a model for understanding Fe-S protein biogenesis in eukaryotes.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
