| Field | Specification |
|---|---|
| Target | |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C11H9Br4N3O2 |
| Purity | |
| Activity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
CK2/ERK8-IN-1 is a dual casein kinase 2 (CK2) and ERK8 (MAPK15, ERK7) inhibitor, with a Ki of 0.25 μM for CK2 and IC50s of 0.50 μM. It also binds to PIM1, HIPK2 (homeodomain-interacting protein kinase 2), and DYRK1A with Kis of 8.65 μM, 15.25 μM, and 11.9 μM, respectively, and it has pro-apoptotic efficacy[1]. It is supplied as a white to off-white solid (C11H9Br4N3O2, MW 534.82) at 99.0% purity.
Physical & Chemical Properties
| CAS Number | 1085822-09-8 |
|---|---|
| Molecular Formula | C11H9Br4N3O2 |
| Molecular Weight | 534.82 g/mol |
| Purity | 99.0% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | O=C(O)CN1C2=C(Br)C(Br)=C(Br)C(Br)=C2N=C1N(C)C |
| Target | CK2, ERK8, PIM1, HIPK2, DYRK1A |
| Signaling Pathway | Cell Cycle/DNA Damage; Stem Cell/Wnt; MAPK/ERK Pathway; JAK/STAT Signaling; Protein Tyrosine Kinase/RTK; Apoptosis |
| Bioactivity Class | Apoptosis |
| Solubility | In Vitro: DMSO: 16.67 mg/mL (31.17 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
|
CK2 0.5 μM (IC50) |
CK2 0.25 μM (Ki) |
ERK8 0.5 μM (IC50) |
PIM1 8.65 μM (Ki) |
HIPK2 15.25 μM (Ki) |
DYRK1A 11.9 μM (Ki) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 16.67 mg/mL (31.17 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | 1.67 mg/mL (3.12 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 1.67 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (16.7 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | 1.67 mg/mL (3.12 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 1.67 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (16.7 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 1.67 mg/mL (3.12 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 1.67 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (16.7 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
In Jurkat cells, CK2/ERK8-IN-1 (Compound K66; 0-50 μM; 24 hours) shows cytotoxic activity[1].
Cell Viability Assay[1]
| Cell Line | Jurkat cells |
|---|---|
| Concentration | 0-50 μM |
| Incubation Time | 24 hours |
| Result | Inhibited cell growth in a dose-dependent manner. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Chloride intracellular channel CLIC3 mediates fibroblast cellular senescence by interacting with ERK7. Commun Biol 2025 Jan 14;8(1):51. PMID: 39809890
