| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | clpP; CBO3231; CLC_3142ATP-dependent Clp protease proteolytic subunit; EC 3.4.21.92; Endopeptidase Clp |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Clostridium botulinum ATP-dependent Clp protease proteolytic subunit protein (1-194AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-clpP antibody raised in Rabbit, conjugated with FITC. Designed to detect clpP in Clostridium botulinum for others research.
Key elements and design rationale
- Immunogen: Recombinant Clostridium botulinum ATP-dependent Clp protease proteolytic subunit protein (1-194AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.
Biological background
clpP (also: clpP, CBO3231, CLC_3142ATP-dependent Clp protease proteolytic subunit, EC 3.4.21.92, Endopeptidase Clp) — studied in Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current clpP annotation relevant to others.
Common research applications
- Refer to product documentation for confirmed application data.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Clostridium botulinum; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
clpP is a protein studied in Clostridium botulinum biology. This polyclonal antibody raised in Rabbit detects clpP in immunoassay applications, IgG isotype.
Reported reactive against Clostridium botulinum. Immunogen from Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A). Cross-reactivity with untested species requires empirical confirmation.
Application data not specified. Consult datasheet or contact BioHippo support.
FITC (Ex490/Em525): direct fluorescence; protect from light; use antifade for sections.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing clpP; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
