| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized powder. |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | DMSO. Centrifuge all product preparations before use (10000 x g for 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 6-Cyano-7-nitroquinoxaline-2,3-dione.
- CAS number: 115066-14-3
- Molecular formula: C9H4N4O4.
- Purity: >98%
- Concentration: 300 nM - 500 µM.
- Form: Lyophilized powder.
- Solubility: DMSO. Centrifuge all product preparations before use (10000 x g for 5 min).
- Reconstitution: DMSO. Centrifuge all product preparations before use (10000 x g for 5 min).
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: AMPA/Kainate receptors
- Source: Synthetic
- Activity: AMPA/kainate receptor antagonist. The concentrations that inhibit 50% of AMPA binding (IC50) for CNQX in dorsal horn neurons and motoneurons are 300 nM-1.3 µM. It was about one-fifth as effective at kainate binding sites1,2. The association of AMPA receptors with TARPs converts CNQX from an antagonist to a weak partial agonist. CNQX binds with high affinity to both high and low affinity AMPA binding sites in rat brain. CNQX is a potent antagonist in electrophysiological responses mediated by non-NMDA receptors. Effects at NMDA receptors are much weaker.
Peptide confirmation
Confirmed by amino acid analysis and mass spectrometry
Scientific background
The first widely used competitive AMPA receptor antagonists were quinoxalinediones (CNQX, DNQX, NBQX), which were highly selective over NMDA receptors but antagonized kainate receptors. CNQX is a potent, competitive AMPA/kainate receptor antagonist. It also acts as an antagonist at the NMDA receptor glycine site.This non-NMDA receptor antagonist inhibits [3H]AMPA binding to quisqualate receptors at submicromolar concentrations. CNQX also selectively blocks the excitatory action of quisqualate and kainate on spinal neurons with little or no effect on that of NMDA.The concentrations that inhibit 50% of [3H]AMPA binding (IC50) for CNQX in dorsal horn neurons is 300 nM1.The association of AMPA receptors with TARP auxiliary subunits converts CNQX from an antagonist to a weak partial agonist. CNQX induces partial domain closure, consistent with the activity of a partial agonist. CNQX blocks both fast AMPA-mediated and slow kainate receptor-mediated mEPSCs2.EC50 values for depression of the monosynaptic ventral root reflex is 1.0 ± 0.3 µM3.Direct binding studies using CNQX as a radioligand show that CNQX binds with high affinity (40 nM) to both high (14 nM) and low (235 nM) affinity AMPA binding sites in rat brain4.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
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