| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Source | Plant — Apocynaceae Tabernaemontana divaricata (Linnaeus) R. Brown ex Roemer & Schultes |
| Molecular weight | |
| Molecular formula | C21H26N2O2 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Coronaridine is an iboga-type alkaloid that inhibits the wnt signaling pathway by decreasing β-catenin expression[1]. It is an indole alkaloid supplied as a white to off-white solid (C21H26N2O2, MW 338.44) at 99.23% purity, sourced from the plant Tabernaemontana divaricata (Apocynaceae).
Physical & Chemical Properties
| CAS Number | 467-77-6 |
|---|---|
| Molecular Formula | C21H26N2O2 |
| Molecular Weight | 338.44 g/mol |
| Purity | 99.23% |
| Appearance | Solid |
| Color | White to off-white |
| Structure Classification | Alkaloids Indole Alkaloids |
| SMILES | COC([C@]12[C@@]3([H])[N@@](CCC4=C2NC5=CC=CC=C45)C[C@@](C[C@@H]3CC)([H])C1)=O |
| Signaling Pathway | Stem Cell/Wnt |
| Initial Source | Plant — Apocynaceae Tabernaemontana divaricata (Linnaeus) R. Brown ex Roemer & Schultes |
| Solubility | In Vitro: DMSO: 50 mg/mL (147.74 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | 4°C, protect from light. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 50 mg/mL (147.74 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); protect from light; avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 1.25 mg/mL (3.69 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 1.25 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (12.5 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
Against non-cancer cells, Coronaridine (0-40 μM; 24 hours) gives IC50 values >40 μM. Against WNT-dependent cells, the IC50 values are 10.4, 11.6 and 24.4 μM for SW480, HCT116 and DLD1 cells, respectively[1]. β-catenin expression is inhibited by Coronaridine (0-40 μM; 24 hours), yet p-β--catenin at Ser33, Ser37, and Thr41, as well as p-β-catenin at Ser 45 [p-b-catenin (S45)], show unchanged protein levels[1]. In whole-cell patch clamp recordings, Catharanthine (1-300 μM) is co-applied with GABA, respectively, at concentrations matching the EC30 value for each receptor subtype. Different GABAARs were potentiated by both congeners in a concentration-dependent manner[2]. Catharanthine, however, begins at higher concentrations to inhibit GABA-activated currents, owing to reduced amplitude and rebound current; the threshold concentration depends on the receptor subtype (e.g., > 30 μM for hα1β2; > 100 μM for hα1β2γ2 and hα2β2γ2). Catharanthine's PAM activity varies with the receptor subtype: hα1β2 (4.6±0.8 μM), >hα2β2γ2 (12.6±3.8 μM), hα1β2γ2 (14.4 ± 4.6 μM)[2].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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EMX1 functions as a tumor inhibitor in spinal cord glioma through transcriptional suppression of WASF2 and inactivation of the Wnt/β-catenin axis. Brain Behav 2022 Jul 18;e2684. PMID: 35849030
