| Field | Specification |
|---|---|
| Mfr No | |
| Assay Type | |
| Product Type | |
| Sample Type(s) | serum |
| Storage | |
| Target |
Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to stimulation of the adrenal cortex by ACTH and is the precursor of aldosterone. Corticosterone is a major indicator of stress and is the major stress steroid produced in non-human mammals. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns.
Assay Principle
Tribioscience’s Corticosterone Competitive ELISA employs the competitive sandwich enzyme immunoassay technique to detect corticosterone concentrations in a variety of samples from plasmid, serum, and cell culture medium.
Kit Contents and Storage Conditions Part # Catalog Number TBS31002-01 TBS31002-05 Cortocosterone Microplate TBS31002A 1x 96-well polystyrene microplate (12 strips of 8 wells) coated with polyclonal antibody 5x 96-well polystyrene microplate (12 strips of 8 wells) coated with polyclonal antibody Corticosterone Standard TBS31002B 1x 0.1mL, Corticosterone Standard (10 ng/mL) 5x 0.1mL, Corticosterone Standard (10 ng/mL) Detecion A TBS31002C 1x 3mL, a corticosterone-peroxidase conjugate in a special stabilizing solution 5x 3mL, A corticosterone-peroxidase conjugate in a special stabilizing solution Detection B TBS31002D 1x 3mL, a polyclonal antibody specific for corticosterone 5x 3mL, a polyclonal antibody specific for corticosterone Assay Diluent TBS31002E 1x 2mL, 5x concentrate Assay Diluent 1x 10mL, 5x concentrate Assay Diluent 10x Wash Buffer TBS3000W 1x 10mL, 10x concentrated solution 1x 50mL, 10x concentrated solution TMB Substrate TBS3000T 1x 12mL, ultra-sensitive TMB substrate 5x 12mL, ultra-sensitive TMB substrate Stop Solution TBS3000S 1x 6mL, 2N sulfuric acid 1x 30mL, 2N sulfuric acid Store the unopened kit between 2-8℃.
Corticosterone Competitive ELISA Kit is a Competitive ELISA for the quantitative determination of Corticosterone. In this competitive ELISA, sample analyte competes with a labeled competitor for a limited number of antibody binding sites, so signal is inversely proportional to analyte concentration.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Corticosterone |
|---|---|
| Sample Type | serum |
| Assay Type | Competitive |
| Test Principle | Competitive ELISA |
| Storage | Store the unopened kit between 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for serum. Other matrices may require additional in-lab validation.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this competitive ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
