| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human CRBN recombinant protein (Position: M1-H378) was used as the immunogen for the CRBN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CRBN Antibody / Cereblon is a anti-CRBN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: CRBN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, FACS, ELISA
Biological background
Cereblon is encoded by the CRBN gene located on human chromosome 3p26.2. The protein consists of 442 amino acids and contains a conserved Lon domain-like region essential for ligand binding and substrate recognition. Within the CRL4 complex, Cereblon acts as a molecular adapter, recruiting neo-substrates such as Ikaros (IKZF1), Aiolos (IKZF3), and CK1alpha in response to drug binding. This interaction forms the basis for therapeutic degradation of disease-associated transcription factors in multiple myeloma and other cancers. Loss-of-function mutations in CRBN have been linked to autosomal recessive intellectual disability, highlighting its importance in neuronal signaling and synaptic plasticity.
The CRBN antibody enables detailed examination of the protein's expression and regulation across tissues. Western blot analysis typically reveals bands near 51 kDa corresponding to full-length Cereblon, while smaller isoforms may be detected in certain tissues. Immunofluorescence shows localization to both the cytoplasm and nucleus, depending on cell type and activation state. Researchers use this antibody to assess CRBN abundance in studies of proteolysis-targeting chimeras (PROTACs), a rapidly growing class of therapeutic agents that harness Cereblon for selective degradation of target proteins.
Beyond oncology, CRBN influences neuronal excitability, metabolic regulation, and circadian rhythm. It has been shown to modulate BK channel activity and AMP-activated protein kinase signaling, linking it to ion channel control and energy metabolism.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
