| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Methylguanidoacetic acid, N-Carbamimidoyl-N-methylglycine |
| Assay Type | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, Plasma |
| Sensitivity | |
| Shipping | |
| Species | |
| Storage | |
| Target |
Scientific Background
Creatinine, a non-protein nitrogenous waste product derived from the non-enzymatic breakdown of phosphocreatine in skeletal muscle, is continuously excreted by the kidneys through glomerular filtration with negligible tubular secretion or reabsorption at normal serum concentrations. This makes serum creatinine a cornerstone biomarker of glomerular filtration rate (GFR) estimation and a standard surrogate for renal function in clinical and preclinical settings.
In neuroscience research, serum creatinine measurements inform systemic health monitoring for rodent cohorts exposed to nephrotoxic pharmacological agents or undergoing chronic disease modeling. Elevated serum creatinine in rodent models correlates with accumulation of uremic toxins that potentiate neuroinflammation and contribute to hippocampal and cortical dysfunction.
Its low intra-individual biological variability (<15% CV) makes creatinine an ideal internal reference for multi-timepoint longitudinal studies. Combined with BUN, GFR estimations can assess renal clearance of neurotoxic metabolites in chronic kidney disease and cardio-renal syndrome research.
Product Description
StressXpress®
The Creatinine Serum Detection Kit (Cat. # SKT-217) from StressMarq Biosciences is a research-grade kit for quantitative Creatinine measurement in Serum, Plasma. This kit employs a direct colorimetric detection principle with colorimetric TMB/spectrophotometric readout on a standard 96-well microplate reader.
Product Specifications
| Analyte / Target | Creatinine |
|---|---|
| Assay Type | Direct Quantitative Assay |
| Detection Method | Colorimetric (microplate spectrophotometry) |
| Sensitivity | 0.085 mg/dl |
| Detection Range | 0.5–4 mg/dl |
| Compatible Matrices | Serum, Plasma |
| Species Reactivity | Human, Mouse, Rabbit, Rat, Sheep |
| Throughput | 91 samples in duplicate |
| Platform | Microplate (96-well) |
| Catalog Number | SKT-217 |
| Storage | 4ºC |
| Trademark | StressXpress® |
Assay Overview
This kit uses a direct colorimetric enzymatic reaction for quantitative Creatinine measurement without the need for sample pre-extraction. A calibrated standard (traceable to NIST reference material where applicable) is provided to construct a standard curve for interpolation. Results are read on a standard microplate spectrophotometer.
Compatible sample matrices include: Serum, Plasma.
Kit Components
| Component # | Description | Quantity / Volume |
|---|---|---|
| SKC-217A | Clear 96-Well Half Area Plates | 2 Plates |
| SKC-217B | Creatinine Standard | 100 µl |
| SKC-217C | Assay Diluent | 6 ml |
| SKC-217D | StressXpress® Creatinine Reagent | 20 ml |
Quality Control & Validation
✓ Peer-Reviewed & Research Validated
Each lot is validated for sensitivity, intra-assay precision (CV <10%), and linearity of recovery. Products from the StressXpress® range cite published peer-reviewed literature for independent validation.
Safety & Regulatory
ADR Classification: Non-hazardous. Shipping: cold-chain (blue ice). Harmonized Tariff Code: 3822.19.0030 (UNSPSC: 12352203). Country of Origin: Canada.
This colorimetric assay is validated for use with: Serum, Plasma. Samples should be diluted with the supplied Assay Diluent or deionized water as specified in the protocol. Lipemic, highly pigmented, or protein-rich samples may require additional dilution to minimize matrix interference.
The kit has a sensitivity of 0.085 mg/dl and a quantification range of 0.5–4 mg/dl. This range is appropriate for typical physiological concentrations. Samples outside the range should be diluted (upper) or concentrated using ultrafiltration (lower).
Total assay time is approximately 30–60 minutes once reagents are prepared. Refer to the Kit Booklet for the detailed protocol and timing requirements.
The kit contains: Clear 96-Well Half Area Plates, Creatinine Standard, Assay Diluent, StressXpress® Creatinine Reagent. All reagents are pre-formulated and lot-matched. A calibrated creatinine or nitrogen standard is included for standard curve generation.
A standard 96-well microplate spectrophotometer capable of reading absorbance at the target wavelength (typically 490–550 nm for colorimetric reactions; see Kit Booklet for exact wavelength) is required. The assay is not compatible with a fluorescence reader.
Urine and tissue culture supernatant samples should be diluted with deionized water or assay diluent as specified in the protocol (typically 1:20 for urine). Serum/plasma samples may be used undiluted or at minimal dilution. No extraction step is required. Sample creatinine values are reported in mg/dL; normalize to urine volume or creatinine concentration as appropriate for your experimental design.
This product may be available in custom quantities, alternative formulations, or with additional quality certifications to meet specific experimental requirements. Contact our scientific team through the BioHippo product page to discuss custom sizing, bulk ordering, and specialized packaging options. Special pricing may be available for volume orders and institutional customers.
