CRISPRa (SAM) Jurkat Cell Line

What it means: The parental cell line is the host in which the transgene or reporter was stably integrated. Jurkat (clone E6-1) (specialized host) is a adherent or suspension cell line selected for this product.

Why it matters:

• Enables consistent transgene expression in a characterized background.
• The parental background determines endogenous receptor expression, growth properties, and media requirements—verify compatibility with your assay conditions.
• Secondary reagents (antibodies, growth media) should be validated for this host species and cell type.

How to interpret here: This product is engineered on a Jurkat (clone E6-1) background. Pathway activity readouts, IC50/EC50 values, and control responses are calibrated for this host. Results may differ in alternative cell backgrounds.

What it means: Species refers to the biological origin of the expressed gene or protein target in this cell line. This product is classified as Human origin.

Why it matters:

• The transgene or expressed protein is of human sequence. This maximizes compatibility with human-specific antibodies, human interactome databases, and clinically relevant pathway contexts.
• Species origin directly affects antibody cross-reactivity, functional assay interpretation, and translational relevance of results.
• Confirm that your detection antibodies, assay controls, and signaling reagents are validated for Human targets.

How to interpret here: The Human designation indicates the sequence and biological context of the expressed construct—not necessarily the host cell species. Check the host cell line chip for the cellular background.

What it means: BSL-1 (Biosafety Level 1) indicates this agent is not known to cause disease in healthy adults and poses minimal risk to laboratory personnel and the environment.

Why it matters:

• Standard microbiological practices apply; no special containment facility is required beyond a clean bench.
• Routine PPE (gloves, lab coat, eye protection) and hand-washing protocols are sufficient.
• Institutional Biosafety Committee (IBC) registration may still be required for genetically modified organisms—consult your IBC before use.

How to interpret here: BSL-1 means this cell line can be handled on an open bench in a standard laboratory. Check your institution's IBC policy, particularly if the construct contains a selectable antibiotic resistance marker or modified genetic elements.

What it means: CRISPR KO Cell Line. A CRISPR KO Cell Line has the target gene disrupted using CRISPR/Cas9 genome editing to create a loss-of-function model validated at the genomic and protein level.

Why it matters:

• Provides an isogenic null background for mechanistic studies, target validation, and off-target screening without confounding effects of overexpression systems.
• Select the correct product type based on whether your experiment requires overexpression, loss-of-function, pathway reporting, or immune effector function.

How to interpret here: This CRISPR KO Cell Line eliminates endogenous expression of the indicated target. Confirm knockout by Western blot and genomic sequencing in your lab before experimental use.

What it means: Liquid Nitrogen storage indicates this cell line must be maintained in vapor-phase or liquid-phase liquid nitrogen (≤-130°C) for long-term preservation of viability and function.

Why it matters:

• Liquid nitrogen cryopreservation halts cellular metabolism and ice crystal formation, preserving cell viability and transgene expression stability for extended periods.
• Storage above -130°C (e.g., -80°C freezer) is not equivalent—cells may lose viability within weeks to months at -80°C.
• Transfer cells from dry-ice shipping to Liquid Nitrogen storage immediately upon receipt to prevent viability loss.

How to interpret here: Liquid Nitrogen storage is required for this product. Access to a liquid nitrogen Dewar or cryogenic storage tank is essential before ordering. Do not store in a standard -80°C freezer as long-term cryostorage.