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Scientific Background
Human CRTAM, also known as Cytotoxic and Regulatory T-Cell Molecule, GenBank Accession No. NM_019604, amino acids 18-286, expressed in a HEK293 expression system. This protein has a C-terminal Avi-Tag fused to the Fc region of Human IgG1 and is enzymatically biotinylated using Avi-tag™ technology. MW = 59 kDa.
Product Description
Mammalian (HEK293) ExpressionRecombinant CRTAM Human protein is produced using a validated Mammalian (HEK293) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | O95727 |
|---|---|
| Expression System | Mammalian (HEK293) |
| Amino Acids / Region | 18-286 |
| Molecular Weight | 59 kDa |
| Formulation | 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol. |
| Storage | At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to … |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
FOR RESEARCH USE ONLY (RUO). Not for diagnostic or therapeutic applications.
This protein was produced in Mammalian (HEK293). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 18-286. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
This protein is suitable for biochemical assays including enzyme kinetics, inhibitor screening, protein binding studies, and drug discovery. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Boles, K., et al., Blood. 2005 Aug; 106(3): 779-86.
- Yeh, J., et al., Cell. 2008 Jan; 132(5):846-859.
