| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
CSL, also known as CBF1 or RBP-Jκ, is the central DNA-binding transcription factor of the canonical Notch signaling pathway. In the absence of Notch activation, CSL functions as a transcriptional repressor at Notch target genes. Upon ligand binding, the Notch intracellular domain (NICD) is released and translocates to the nucleus, where it forms a ternary complex with CSL and Mastermind-like coactivators to activate transcription. Notch signaling, transmitted through Notch1-4 receptors, governs cell-fate decisions, differentiation, and tissue patterning throughout development, and its dysregulation contributes to cancer and developmental disorders. Because CSL integrates input from all four receptors, CSL reporters give a broad readout of pan-Notch activity.
Product Description & Applications
The CSL Reporter Lentivirus is a transcription factor reporter system for robust detection of pan-Notch (Notch1-4) transcriptional activity in mammalian cells. Tandem Notch response elements, coupled to a minimal TATA promoter and upstream enhancer, drive a fluorescent or luminescent reporter in response to NICD-CSL complex formation. Unlike Notch1-preferential reporters, the CSL design captures a broader response, including signaling from the less potent Notch3. Stable lentiviral integration enables generation of polyclonal reporter cell lines for analysis by fluorescence microscopy, flow cytometry, or luminometry. The system is applied to study Notch signaling in development, differentiation, and cancer. Particles are purified by PEG precipitation and sucrose gradient centrifugation, supporting efficient transduction of difficult-to-transfect cells, including primary and cryopreserved cultures.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the CSL (CBF1/RBP-Jk) transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
