CT26 cell

SKU:BHC11101426
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Overview
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CT26 cell is a cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Mouse
Disease model Adenocarcinoma
Growth Properties Adherent
Tissue Colon
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Catalog no. Size
305229 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305229
Species Mouse
CT26 is a widely utilized murine colon carcinoma cell line derived from BALB/c mice. These cells are characterized by their epithelial-like morphology and have been extensively used in cancer research, particularly in studies focusing on tumor immunology and the development of cancer therapies. The CT26 cell line is valuable due to its high tumorigenic potential and ability to form tumors when implanted in syngeneic mice, making it an excellent model for investigating the mechanisms of tumor growth and metastasis in a controlled environment. Research involving CT26 cells has provided critical insights into the immune system's response to tumors, aiding in the development of novel immunotherapeutic approaches. These cells are often used in conjunction with immunomodulatory agents to assess the efficacy of potential treatments and to study the interactions between cancer cells and the immune system. The CT26 cell line's compatibility with various genetic manipulation techniques further enhances its utility in exploring the molecular underpinnings of cancer and testing new therapeutic strategies. Overall, the CT26 cell line is a cornerstone in preclinical cancer research, contributing to the understanding of colorectal cancer biology and the advancement of therapeutic interventions. Its relevance in immunotherapy studies underscores its importance in the ongoing efforts to develop effective cancer treatments. Due to its robust nature and well-documented characteristics, CT26 continues to be a preferred model in oncology research.

SKU:BHC11101426

Tumorigenic: Yes, in BALB/c mice

  • cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
  • supplements: Supplement the medium with 10% FBS
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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