| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | (Ala-IL-8)77 antibody; (Ser-IL-8)72 antibody; 9E3 antibody; Beta thromboglobulin like protein antibody; C-X-C motif chemokine 8 antibody; CEF-4 antibody; chemokine; CXC motif; ligand 8 antibody; CXCL8 antibody; Emoctakin antibody; GCP-1 antibody; GCP/IL-8 protein I antibody; GCP/IL-8 protein II antibody; GCP/IL-8 protein III antibody; GCP/IL-8 protein IV antibody; GCP/IL-8 protein V antibody; GCP/IL-8 protein VI antibody; GCP1 antibody; Granulocyte chemotactic protein 1 antibody; IL-8 antibody; IL-8(1-77) antibody; IL-8(9-77) antibody; IL8 antibody; IL8/NAP1 form I antibody; IL8/NAP1 form II antibody; IL8/NAP1 form III antibody; IL8/NAP1 form IV antibody; IL8/NAP1 form V antibody; IL8/NAP1 form VI antibody; IL8_HUMAN antibody; Inteleukin 8 antibody; LECT antibody; LUCT antibody; Lymphocyte-derived neutrophil-activating factor antibody; LYNAP antibody; MDNCF antibody; MDNCF-b antibody; MDNCF-c antibody; MONAP antibody; Monocyte derived neutrophil activating peptide antibody; Monocyte derived neutrophil chemotactic factor antibody; Monocyte-derived neutrophil chemotactic factor antibody; Monocyte-derived neutrophil-activating peptide antibody; NAF antibody; NAP 1 antibody; NAP-1 antibody; NAP1 antibody; Neutrophil activating peptide 1 antibody; Neutrophil activating protein 1 antibody; Neutrophil-activating factor antibody; Neutrophil-activating protein 1 antibody; Protein 3 10C antibody; Protein 3-10C antibody; SCYB 8 antibody; SCYB8 antibody; Small inducible cytokine subfamily B member 8 antibody; T cell chemotactic factor antibody; T-cell chemotactic factor antibody; TSG 1 antibody; TSG1 antibody |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human CXCL8 protein |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a monoclonal anti-CXCL8 antibody raised in Human, with confirmed utility in ELISA. It is designed to detect CXCL8 protein in Human and supports researchers working in others contexts.
Key elements and design rationale
- Immunogen: Recombinant Human CXCL8 protein — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Human): Requires anti-human-IgG secondary reagents for indirect detection.
- Monoclonal format: Single-epitope binding provides high specificity and lot-to-lot reproducibility. Confirm epitope accessibility under your assay conditions.
- Isotype (IgG2): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Affinity chromatography): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
CXCL8 (also referred to as (Ala-IL-8)77 antibody, (Ser-IL-8)72 antibody, 9E3 antibody, Beta thromboglobulin like protein antibody, C-X-C motif chemokine 8 antibody, CEF-4 antibody, chemokine, CXC motif, ligand 8 antibody, CXCL8 antibody, Emoctakin antibody, GCP-1 antibody, GCP/IL-8 protein I antibody, GCP/IL-8 protein II antibody, GCP/IL-8 protein III antibody, GCP/IL-8 protein IV antibody, GCP/IL-8 protein V antibody, GCP/IL-8 protein VI antibody, GCP1 antibody, Granulocyte chemotactic protein 1 antibody, IL-8 antibody, IL-8(1-77) antibody, IL-8(9-77) antibody, IL8 antibody, IL8/NAP1 form I antibody, IL8/NAP1 form II antibody, IL8/NAP1 form III antibody, IL8/NAP1 form IV antibody, IL8/NAP1 form V antibody, IL8/NAP1 form VI antibody, IL8_HUMAN antibody, Inteleukin 8 antibody, LECT antibody, LUCT antibody, Lymphocyte-derived neutrophil-activating factor antibody, LYNAP antibody, MDNCF antibody, MDNCF-b antibody, MDNCF-c antibody, MONAP antibody, Monocyte derived neutrophil activating peptide antibody, Monocyte derived neutrophil chemotactic factor antibody, Monocyte-derived neutrophil chemotactic factor antibody, Monocyte-derived neutrophil-activating peptide antibody, NAF antibody, NAP 1 antibody, NAP-1 antibody, NAP1 antibody, Neutrophil activating peptide 1 antibody, Neutrophil activating protein 1 antibody, Neutrophil-activating factor antibody, Neutrophil-activating protein 1 antibody, Protein 3 10C antibody, Protein 3-10C antibody, SCYB 8 antibody, SCYB8 antibody, Small inducible cytokine subfamily B member 8 antibody, T cell chemotactic factor antibody, T-cell chemotactic factor antibody, TSG 1 antibody, TSG1 antibody) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of CXCL8 biology in others.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG2 from Human) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Confirm the clone does not cross-react with related family members under your assay conditions.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a recombinant monoclonal antibody expressed in HEK293F cells.
CXCL8 is a protein target in Human biology. This monoclonal antibody raised in Human is designed to detect CXCL8 in ELISA applications, with IgG2 isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG2 antibody requires a compatible secondary. Select anti-human IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG2.
(1) Isotype control — IgG2 from Human at matching concentration; (2) Positive control — known CXCL8-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
