| Field | Specification |
|---|---|
| Target | |
| Host | |
| Reactivity | |
| Immunogen | Human IgG, whole molecule |
| Conjugate | |
| Purification | |
| Concentration | |
| Storage | |
| Storage buffer | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This Cy5-conjugated donkey anti-Human IgG [H&L] secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography and solid-phase adsorption to minimize cross-reactivity. With emission at 667 nm, it is suited to far-red fluorescence detection of human IgG in multiplexed imaging and immunoassay formats.
Background
Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins
Immunogen
Human IgG, whole molecule
Specifications
| Host | Donkey |
|---|---|
| Conjugate | Cy5 |
| Emission Wavelength | 667 nm |
| Reactivity | Human |
| Immunogen | Human IgG, whole molecule |
| Purification | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins. |
| Concentration | 1.0 mg/ml |
| Storage Buffer | 0.01% (w/v) Sodium Azide |
| Stabilizer | 10 mg/ml BSA IgG and Protease free |
| Storage | For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of restoration. |
| Shipping | Ice Pack |
Reactivity Notes
Reactivity: Human. Cross-reactivity with other species has not been fully characterized; validate in your system before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. Contains sodium azide where stated; handle with standard laboratory precautions.
Related Products
Explore related antibodies and detection reagents for Human IgG research from Applied Biological Materials (abm) Inc., or contact us for alternative conjugates and formats.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
