| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
Product Overview
DB Antigen retrieval solution, pH9, 10xconcentrated is an immunohistochemistry reagent from DB Biotech.
DB Antigen retrieval solution pH9 is designed for the application in immunohistochemistry to optimize the antibody performance with heat induced antigen retrieval on formalin-fixed, paraffin-embedded tissues.
Composition & Storage
| Preservative | 0.5% NaN3. |
|---|---|
| Storage | 4-8 °C. |
Procedure
Prepare a working solution by diluting the concentrate DB RETRIS 1:10 in distilled water. The ready to use solution can be used for up to 3 months if stored at 4-8 °C.
Safety & Handling
For Research Use Only (RUO). Consult the Safety Data Sheet before use.
pH 9 (Tris-EDTA) retrieval is particularly effective for nuclear antigens such as Ki-67, p53, histone modifications, and certain transcription factors that are suboptimally retrieved under pH 6 conditions. It also improves retrieval for some cytoplasmic and membrane proteins that have strong crosslinking in the nuclear region. Always empirically test both pH 6 and pH 9 for new antibody-tissue combinations.
While pH 9 retrieval is generally well-tolerated by most tissue types, some delicate tissues (e.g., brain, skin, embryonic tissue) may show section detachment at high temperature with alkaline buffer. Ensure sections are mounted on adhesive-coated slides (e.g., SuperFrost Plus) and allow adequate drying time before deparaffinization. Use a retrieval vessel that allows controlled temperature maintenance.
BioHippo partners with leading life science suppliers to offer flexible purchasing and support options. Contact our team to discuss the following add-on services.
- Bulk & Volume Pricing: Reduced per-unit pricing for multi-unit or project-scale orders. Submit a quote request for a customized offer.
- Expedited Fulfillment: Priority order handling may be available for time-sensitive projects. Confirm availability before ordering.
- Technical Consultation: Our scientific team provides protocol guidance and troubleshooting at no additional charge.
- Custom Configurations: Alternative formulations, pack sizes, or kit configurations may be available upon request.
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