| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
Product Overview
DB Primary Antibody diluent is an immunohistochemistry reagent from DB Biotech.
DB Antibody diluent is designed for the application in immunohistochemistry. The reagent is in a ready-to-use format and is used to dilute the concentrated primary antibodies. It is designed in order to optimize the antibody performance and reduce the possible nonspecific background staining.
Composition & Storage
| Preservative | 0.05% NaN3. |
|---|---|
| Stabilizer | 10 mg/ml BSA. |
| Storage | 4-8 °C. |
Procedure
Based on suggested dilutions, add the concentrated antibody directly to the diluent and mix thoroughly.
Safety & Handling
For Research Use Only (RUO). Consult the Safety Data Sheet before use.
This diluent is formulated for use with primary antibodies of all major classes (IgG, IgM) from common host species including rabbit, mouse, goat, and rat. It is suitable for both polyclonal and monoclonal primary antibodies used in chromogenic and fluorescent IHC protocols on FFPE tissue sections.
Yes. The formulation contains blocking agents that occupy non-specific binding sites on tissue sections, helping to reduce background staining without compromising specific signal. Optimal dilution of the primary antibody in this diluent should still be determined by titration, as antibody-specific characteristics also contribute to background.
The diluent is optimized primarily for FFPE tissue sections. For frozen sections, compatibility should be evaluated empirically, as membrane antigen accessibility and tissue adhesion requirements differ. Consult the antibody manufacturer's recommended diluent for frozen section protocols.
BioHippo partners with leading life science suppliers to offer flexible purchasing and support options. Contact our team to discuss the following add-on services.
- Bulk & Volume Pricing: Reduced per-unit pricing for multi-unit or project-scale orders. Submit a quote request for a customized offer.
- Expedited Fulfillment: Priority order handling may be available for time-sensitive projects. Confirm availability before ordering.
- Technical Consultation: Our scientific team provides protocol guidance and troubleshooting at no additional charge.
- Custom Configurations: Alternative formulations, pack sizes, or kit configurations may be available upon request.
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