| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C44H42N4O6 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
DBCO-PEG2-DBCO is a click chemistry reagent bearing DBCO groups; it is a PEG linker with two terminal DBCO groups, which are used for copper-free click chemistry reactions owing to their strain-promoted high reactivity[1]. It is reagent grade, for research use only, and is supplied as a white to yellow solid (C44H42N4O6, MW 722.83) at 95.43% purity.
Physical & Chemical Properties
| CAS Number | 2639395-48-3 |
|---|---|
| Molecular Formula | C44H42N4O6 |
| Molecular Weight | 722.83 g/mol |
| Purity | 95.43% |
| Appearance | Solid |
| Color | White to yellow |
| SMILES | O=C(CCC(N1C2=C(C#CC3=C(C1)C=CC=C3)C=CC=C2)=O)NCCOCCOCCNC(CCC(N4C5=C(C#CC6=C(C4)C=CC=C6)C=CC=C5)=O)=O |
| Signaling Pathway | Antibody-Drug Conjugate/ADC Related |
| Solubility | In Vitro: DMSO: 100 mg/mL (138.35 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 100 mg/mL (138.35 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 2.5 mg/mL (3.46 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | 2.5 mg/mL (3.46 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 2.5 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Data provided by the manufacturer.
In Vitro
DBCO-PEG2-DBCO is a click chemistry reagent that contains DBCO groups. Because of their strain-promoted high energy, DBCO groups are commonly used in copper-free click chemistry reactions[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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