| Field | Specification |
|---|---|
| Target | |
| Alternative names | 2',3'-Dideoxyadenosine 5'-triphosphate |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C10H16N5O11P3 |
| SMILES | |
| Form | Liquid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
ddATP, also known as 2',3'-Dideoxyadenosine 5'-triphosphate, is an active metabolite of 2',3'-dideoxyinosine that acts as a chain-elongating inhibitor of DNA polymerase. It can be used in the Sanger method of DNA sequencing and in research on virus infection[1][2][4]. It is supplied as a colorless to light yellow liquid (C10H16N5O11P3, MW 475.18).
Physical & Chemical Properties
| CAS Number | 24027-80-3 |
|---|---|
| Molecular Formula | C10H16N5O11P3 |
| Molecular Weight | 475.18 g/mol |
| Appearance | Liquid |
| Color | Colorless to light yellow |
| SMILES | NC1=NC=NC2=C1N=CN2[C@H]3CC[C@H](O3)COP(O)(OP(OP(O)(O)=O)(O)=O)=O |
| Target | DNA Polymerase |
| Signaling Pathway | Cell Cycle/DNA Damage; Anti-infection |
| Storage | Solution, -20°C, 2 years. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
ddATP (0.05-50 μM, 30 min) blocks adenovirus DNA synthesis in isolated nuclei from adenovirus-infected KB cells[1]. In DSB (DNA double-strand breaks) oocytes, ddATP (100 μM, 5 h) lowers the number of γH2A.X foci[3]. ddATP inhibits HIV replication through inhibition of HIV reverse transcriptase[5].
Immunofluorescence[3]
| Cell Line | DSB oocytes |
|---|---|
| Concentration | 100 μM |
| Incubation Time | 5 h |
| Result | Suppressed the mtDNA replication without fully suppressed the nuclear DNA replication. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Improved precision, sensitivity, and adaptability of Ordered Two-Template Relay cDNA library preparation for RNA sequencing. RNA 2025 Jan 22;31(2):224-244. PMID: 39626888
