| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C25H29N5O4S |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
DDO-5936 is a potent, specific inhibitor of the Hsp90-Cdc37 protein-protein interaction (PPI) that can be used for colorectal cancer research[1]. It is supplied as a white to yellow solid (C25H29N5O4S, MW 495.59) at 98.33% purity.
Physical & Chemical Properties
| CAS Number | 2355377-13-6 |
|---|---|
| Molecular Formula | C25H29N5O4S |
| Molecular Weight | 495.59 g/mol |
| Purity | 98.33% |
| Appearance | Solid |
| Color | White to yellow |
| SMILES | O=C(CN(S(=O)(C1=C(C)C=C(C)C=C1C)=O)C2=CC=C(NC3=NC(N4CCCC4)=NC=C3)C=C2)O |
| Signaling Pathway | Cell Cycle/DNA Damage; Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 12.5 mg/mL (25.22 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 12.5 mg/mL (25.22 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 1.25 mg/mL (2.52 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 1.25 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (12.5 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Data provided by the manufacturer.
In Vitro
DDO-5936 inhibits the Hsp90-Cdc37 PPI with potency and specificity, acting by binding to a critical site on Hsp90 that involves Glu47[1].
In Vivo
An effect is seen with DDO-5936 (p.o., 0~80 mg/kg) in the high dose group[1]. The compound is well tolerated, as no serious weight loss occurs. In the high dose groups, tumor cells in the xenografts decrease significantly. Oral efficiency of DDO-5936 is limited[1].
| Animal Model | Mice[1] |
|---|---|
| Dosage | 0~80 mg/kg |
| Administration | P.o. |
| Result | Showed a moderate effect at the high dose group. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Discovery of new Hsp90-Cdc37 protein-protein interaction inhibitors: in silico screening and optimization of anticancer activity. RSC Adv 2024 Sep 5;14(39):28347-28375. PMID: 39239280
