| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-dependent RNA helicase DDX19B; DBP5; Dbp5; yeast; homolog of; DD19B_HUMAN; DDX19; DDX19B; DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B; DEAD box protein 19B; DEAD box RNA helicase DEAD5; DEAD-box protein 5; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5; yeast; homolog); DEAD/H BOX 19; helicase DEAD5; RNAh; TDBP; yeast Dbp5 homolog |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from internal of Human DDX19B. |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-DDX19B antibody raised in Rabbit, with confirmed utility in ELISA, WB. It is designed to detect DDX19B protein in Human, Mouse and supports researchers working in signal transduction contexts.
Key elements and design rationale
- Immunogen: Synthesized peptide derived from internal of Human DDX19B. — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Antigen affinity purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
Biological background
DDX19B (also referred to as ATP-dependent RNA helicase DDX19B, DBP5, Dbp5, yeast, homolog of, DD19B_HUMAN, DDX19, DDX19B, DEAD (Asp-Glu-Ala-Asp) box polypeptide 19B, DEAD box protein 19B, DEAD box RNA helicase DEAD5, DEAD-box protein 5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 19 (Dbp5, yeast, homolog), DEAD/H BOX 19, helicase DEAD5, RNAh, TDBP, yeast Dbp5 homolog) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of DDX19B biology in signal transduction.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
- WB: Confirms target molecular weight and distinguishes isoforms under denaturing conditions.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human, Mouse. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
DDX19B is a protein target in Human, Mouse biology. This polyclonal antibody raised in Rabbit is designed to detect DDX19B in ELISA, WB applications, with IgG isotype.
Reported reactive against Human, Mouse. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA, WB. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
This non-conjugated IgG antibody requires a compatible secondary. Select anti-rabbit IgG secondary conjugated to your preferred reporter (HRP, AP, fluorophore, or biotin), matched to IgG.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known DDX19B-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
