| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Hygromycin, Puromycin, RFP (constitutively expressed), Zeocin |
| Shipping | |
| Species |
Background
Dectin-1 is a C-type lectin receptor expressed on dendritic cells, macrophages, neutrophils, and other myeloid cells, where it serves as a key pattern recognition receptor of the innate immune system. It recognizes beta-glucans, carbohydrate components of fungal cell walls, and signals through its immunoreceptor tyrosine-based activation-like motif and the Syk kinase pathway. This signaling activates the transcription factor NF-κB, driving the production of pro-inflammatory cytokines, reactive oxygen species, and other effectors of antifungal defense, and helping to shape adaptive immunity, including Th17 responses. Dectin-1 is studied for its central role in antifungal immunity and inflammation and as a target for immunomodulatory and vaccine adjuvant strategies.
Product Description & Applications
The DECTIN1/NF-κB Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human Dectin-1 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable reporter cell line that responds quantitatively to Dectin-1 engagement, for example by fungal beta-glucans, with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media for kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of antifungal immunity, inflammatory signaling, and Dectin-1-targeting therapeutics in primary and difficult-to-transfect cells.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human DECTIN1 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
