DECTIN2/NFκB Reporter Lentivirus

Background

Dectin-2 is a C-type lectin receptor expressed on dendritic cells, macrophages, and other myeloid cells, where it functions as a pattern recognition receptor of the innate immune system. It recognizes high-mannose structures and alpha-mannans on the cell walls of fungi such as Candida and Malassezia species. Ligand binding triggers signaling through an associated adaptor and the Syk kinase pathway, leading to activation of the transcription factor NF-κB. NF-κB then drives the expression of pro-inflammatory cytokines and other genes that coordinate antifungal defense and shape adaptive immune responses, including Th17 differentiation. Dectin-2 signaling is studied for its roles in antifungal immunity, inflammation, and as a target for immunomodulatory therapeutics.

Product Description & Applications

The DECTIN2/NF-κB Reporter Lentivirus is an immunotherapy reporter system supplied as a two-vial set. A receptor lentivirus drives constitutive expression of human Dectin-2 under a constitutive promoter with antibiotic selection, while a reporter lentivirus carries tandem NF-κB response elements driving a dual reporter combining GFP and secreted Gaussia luciferase. Sequential transduction and selection generates a dual-stable reporter cell line that responds quantitatively to Dectin-2 engagement, for example by fungal mannans, with a combined fluorescent and bioluminescent readout. Secreted Gaussia luciferase accumulates in conditioned media for kinetic sampling without cell lysis. Supplied as high-titer, VSV-G pseudotyped third-generation particles purified by PEG precipitation and sucrose gradient centrifugation, the system supports studies of antifungal immunity, inflammatory signaling, and Dectin-2-targeting therapeutics in primary and difficult-to-transfect cells.

About This Product

This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human DECTIN2 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.

Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.