| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neutrophil defensin 4; Defensin; alpha 4; HNP-4; HP-4; DEFA4; DEF4; α-defensin 4 |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide, corresponding to the N-terminus of Human Defensin α4. |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Human neutrophil alpha-defensins (also designated HNPs) are small, cationic,cysteine-rich antimicrobial proteins that play important roles in innate immunity against infectious microbes such as bacteria, fungi and enveloped viruses. Alpha-defensins are synthesized as inactive precursors and are activated by proteolytic cleavage by MMP-7. Paneth cells in small intestinal crypts secrete the alpha-defensins, which are also termed cryptidins in mice.The cryptidin-4 gene is unique among the Paneth cell alpha defensins in that it is inactive in the duodenum, but expressed at maximal levels in the distal small bowel.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes Defensin α4. Defensin α4 (D27) polyclonal antibody detects endogenous levels of Defensin α4 protein.
Validated Applications
| Applications | Immunohistochemistry |
|---|---|
| IHC | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic peptide, corresponding to the N-terminus of Human Defensin α4.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide, corresponding to the N-terminus of Human Defensin α4. |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Target MW | ~ 11 kDa |
| Swiss-Prot | P12838 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Defensin α4 research from Bioworld Technology Inc, or contact us for alternative formats.
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