DHA Kinase Polyclonal Antibody

SKU:BHA12105116
Overview
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DAK-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunohistochemistry, ELISA in Human, Mouse, Rat samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
Molecular Weight ~59.0 kDa
UniProt Q3LXA3
Gene ID Human: 26007; Mouse: 225913; Rat: 361730
Applications 3 validated
Options selector
Catalog no. Size
BT-AP02590-20UL 20 uL
BT-AP02590-50UL 50 uL
BT-AP02590-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-AP02590
Alternative Names DAK; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase; cyclizing
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 26007; Mouse: 225913; Rat: 361730
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human DAK. AA range:91-140
Isotype
  • IgG
Molecular Weight ~59.0 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Rat
Storage -20°C for 1 year
Target DAK

Product Overview

Primary Antibody

This antibody targets DAK and detects endogenous levels of DAK protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target DAK
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Molecular Weight ~59.0 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from human DAK. AA range:91-140
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 26007; Mouse: 225913; Rat: 361730
UniProt Q3LXA3 (Human: Q3LXA3; Mouse: Q8VC30; Rat: Q4KLZ6)

Scientific Background

TKFC is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of TKFC phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined.

Alternative Names

DAK; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase; cyclizing

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetDAK
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Molecular Weight~59.0 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from human DAK. AA range:91-140
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 26007; Mouse: 225913; Rat: 361730
UniProtQ3LXA3 (Human: Q3LXA3; Mouse: Q8VC30; Rat: Q4KLZ6)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

TKFC is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of TKFC phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been identified, but the full-length nature of only one has been determined.

Alternative Names

DAK; Bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase; cyclizing

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of DAK?
The predicted molecular weight of DAK is ~59.0 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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