| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | ATP dependent RNA helicase #46; ATP-dependent RNA helicase #46; DBP1; DDX15; DEADH box 15; DEAH (Asp Glu Ala His) box polypeptide 15; DEAH box polypeptide 15; DEAH box protein 15; DHX15; DHX15_HUMAN; HRH2; Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15; PRP43; PrPp43p; RNA helicase 2 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 protein (371-502AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This polyclonal anti-DHX15 antibody raised in Rabbit, conjugated with FITC. Designed to detect DHX15 in Human for epigenetics and nuclear signaling research.
Key elements and design rationale
- Immunogen: Recombinant Human Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15 protein (371-502AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.
Biological background
DHX15 (also: ATP dependent RNA helicase #46, ATP-dependent RNA helicase #46, DBP1, DDX15, DEADH box 15, DEAH (Asp Glu Ala His) box polypeptide 15, DEAH box polypeptide 15, DEAH box protein 15, DHX15, DHX15_HUMAN, HRH2, Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX15, PRP43, PrPp43p, RNA helicase 2) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current DHX15 annotation relevant to epigenetics and nuclear signaling.
Common research applications
- Refer to product documentation for confirmed application data.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
DHX15 is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects DHX15 in immunoassay applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Application data not specified. Consult datasheet or contact BioHippo support.
FITC (Ex490/Em525): direct fluorescence; protect from light; use antifade for sections.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing DHX15; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
