| Field | Specification |
|---|---|
| Target | |
| Alternative names | ECO-4601; TLN-4601; BU 4664L |
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C28H34N2O4 |
| Activity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Diazepinomicin, also known as ECO-4601, TLN-4601, and BU 4664L, is an anticancer and antibacterial agent that can be produced by a Micromonospora strain. It induces apoptosis and inhibits the proteases Rhodesain and Cathepsin L with an IC50 of 70-90 μM, while also showing anti-inflammatory and anti-tumor activity, including activity against hepatocellular carcinoma. The compound displays antiparasitic activity against trypomastigote forms of Trypanosoma brucei (IC50 13.5 μM) and moderate antibacterial activity against certain Gram-positive bacteria (MIC ≈ 32 μg/mL)[1][2]. It is supplied as an off-white to gray solid (C28H34N2O4, MW 462.58).
Physical & Chemical Properties
| CAS Number | 733035-26-2 |
|---|---|
| Molecular Formula | C28H34N2O4 |
| Molecular Weight | 462.58 g/mol |
| Appearance | Solid |
| Color | Off-white to gray |
| SMILES | O=C1C2=CC=CC(O)=C2NC3=C(O)C=C(O)C=C3N1C/C=C(C)/CC/C=C(C)/CC/C=C(C)\C |
| Target | Trypanosoma, cathepsin L |
| Signaling Pathway | Apoptosis; Anti-infection; Metabolic Enzyme/Protease |
| Bioactivity Class | Microbial Metabolite |
| Storage | -20°C, protect from light, stored under nitrogen. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target[1]
|
cathepsin L 70-90 μM (IC50) |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
[1]. Rambabu V, et al. In vitro cytotoxicity perspective of diazepinomicin (ECO-4601) on human hepatoma cell line (HEPG2)[J]. Biomedicine & Aging Pathology, 2014, 4(1): 65-70.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
In hepatocellular carcinoma HepG2 cells, Diazepinomicin (1-25 μM; 24-72 h) has dose-dependent antiproliferative activity[1]. Diazepinomicin shows moderate antibacterial activity against specific Gram-positive bacteria, with an MIC of about 32 μg/mL[2].
Apoptosis Analysis[1]
| Cell Line | human hepatoma HepG2 cells |
|---|---|
| Concentration | 10-15 μM |
| Incubation Time | 24 h |
| Result | Induced clumping of cells with slight distortion, as well as very condensed and fragmented nuclei (hallmark features of apoptosis). Induced condensed nuclei and apoptotic bodies, characteristic of apoptotic cells. Induced DNA fragmentation, visualized as characteristic DNA ladder bands on agarose gel electrophoresis. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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