Diethyl azodicarboxylate

SKU:BHB20421129
Overview
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Diethyl azodicarboxylate (CAS 4143-61-7) is a small molecule compound supplied as a solid powder by TargetMol. For biochemical profiling and cell-based assay applications.
Activity Small Molecule Compound
CAS No. 4143-61-7
Molecular Weight 174.15 g/mol
Form Solid
Shipping Room Temperature
Options selector
Catalog no. Size
T31445-100MG 100 mg
T31445-500MG 500 mg
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 100 mg, 500 mg
  • Lead time: In Stock at Manufacturer — options listed in "Availability Content"; other statuses may take longer.
  • Storage: −20 °C
  • Shipping: Room temperature (RT) shipment.
  • Upon receipt: Store at −20 °C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No T31445
Activity
  • Small Molecule Compound
Alternative Names DEAD
Cas No. 4143-61-7
Molecular Weight 174.15
Product Type
  • Small Molecule Inhibitor
Signaling Pathway Others
SMILES C(/N=N/C(OCC)=O)(OCC)=O
Target Others

Compound Overview

Diethyl azodicarboxylate (CAS 4143-61-7) is a research-grade small molecule supplied by TargetMol.

Alternative names: DEAD

Physical Properties

CAS Number 4143-61-7
Molecular Formula C6H10N2O4
Molecular Weight 174.15 g/mol
SMILES C(/N=N/C(OCC)=O)(OCC)=O
Form Solid
Shipping Room Temperature (RT)

Biological Activity

Diethyl azodicarboxylate, conventionally abbreviated as DEAD and sometimes as DEADCAT, is an organic compound with the structural formula CH3CH2O2CN=NCO2CH2CH3. Its molecular structure consists of a central azo functional group, RN=NR, flanked by two ethyl ester groups. This orange-red liquid is a valuable reagent but also quite dangerous and explodes upon heating.

Safety & Regulatory

For Research Use Only (RUO). Not for therapeutic, diagnostic, or clinical use. Wear appropriate PPE and consult the Safety Data Sheet (SDS) prior to handling.

What should I do if the products in powder form adhere to the vial tightly?

Centrifuge the closed vial at 3000 rpm for 1–2 minutes to consolidate the powder at the bottom. For electrostatically charged powders, briefly warm the vial to room temperature and allow it to equilibrate before opening. Then add the appropriate solvent directly onto the powder and vortex before sonicating. Avoid tapping the vial vigorously, as this can generate fine powder aerosol.

what frequency should sonication be used?

Use a bath-type ultrasonic cleaner at standard cleaning frequency (37–40 kHz). Bath sonication is gentler than a probe sonicator and avoids sample overheating. Sonicate in 5–10 second intervals, checking solubility between pulses, for no more than 2–3 minutes total. Warm the bath to 37 °C if solubility remains poor.

Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.

  • Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
  • Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
  • Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
  • QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
  • Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity

To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).

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Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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