| Field | Specification |
|---|---|
| Target | |
| Alternative names | 2810028N01Rik; bA555G22; Dis3; DIS3 homolog; exosome endoribonuclease and 3'-5' exoribonuclease; DIS3 mitotic control homolog (S. cerevisiae); DIS3 mitotic control homolog; DIS3 mitotic control; S. cerevisiae; homolog of; dis3p; EXOSC11; Exosome complex exonuclease RRP44; exosome component 11; KIAA1008; Mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA processing protein 44; Ribosomal RNA processing protein 44; S. cerevisiae; homolog of; Ribosomal RNA-processing protein 44; RP11-342J4.3; RRP44; RRP44_HUMAN |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human Exosome complex exonuclease RRP44 protein (30-187AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-DIS3 antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect DIS3 in Human for epigenetics and nuclear signaling research.
Key elements and design rationale
- Immunogen: Recombinant Human Exosome complex exonuclease RRP44 protein (30-187AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- Biotin: Streptavidin/avidin amplification; block endogenous biotin in biotin-rich samples.
Biological background
DIS3 (also: 2810028N01Rik, bA555G22, Dis3, DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease, DIS3 mitotic control homolog (S. cerevisiae), DIS3 mitotic control homolog, DIS3 mitotic control, S. cerevisiae, homolog of, dis3p, EXOSC11, Exosome complex exonuclease RRP44, exosome component 11, KIAA1008, Mitotic control protein dis3 homolog, Protein DIS3 homolog, Ribosomal RNA processing protein 44, Ribosomal RNA processing protein 44, S. cerevisiae, homolog of, Ribosomal RNA-processing protein 44, RP11-342J4.3, RRP44, RRP44_HUMAN) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current DIS3 annotation relevant to epigenetics and nuclear signaling.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
DIS3 is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects DIS3 in ELISA applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Biotin: streptavidin/avidin amplification. Block endogenous biotin in biotin-rich samples.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing DIS3; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
