| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C46H44F2N8 |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
DIZ-3 is a selective, multimeric G4 ligand designed using a G4-ligand-dimerizing strategy. It intercalates into the G4-G4 interface to stabilize the higher-order structure, inducing cell cycle arrest and apoptosis and thereby inhibiting cell proliferation in alternative lengthening of telomere (ALT) cancer cells[1]. It has the molecular formula C46H44F2N8 (MW 746.89).
Physical & Chemical Properties
| CAS Number | 2675490-72-7 |
|---|---|
| Molecular Formula | C46H44F2N8 |
| Molecular Weight | 746.89 g/mol |
| SMILES | CN1CCN(CC1)C2=CC=C(C=C2)C3=C(NC(C4=CC=C(C=C4)C5=NC(C6=CC=C(C=C6)F)=C(N5)C7=CC=C(C=C7)N8CCN(CC8)C)=N3)C9=CC=C(C=C9)F |
| Signaling Pathway | Cell Cycle/DNA Damage |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
DIZ-3 (0-40 μM; 24 hours) inhibits proliferation in an ALT cancer cell line[1]. At 0.6-2.5 μM for 24 hours, DIZ-3 induces U2OS cell cycle arrest and apoptosis in ALT cancer cells[1]. DIZ-3 (0.12, 0.25, 0.5 μM; 7 days) produces effects in colony formation and wound healing assays performed in U2OS cells[1]. U2OS cell migration is significantly inhibited by DIZ-3 (0.12, 0.25, 0.5 μM; 24 h)[1].
Cell Proliferation Assay[1]
| Cell Line | Human bone osteosarcoma U2OS cells, normal BJ fibroblasts |
|---|---|
| Concentration | 0-40 μM |
| Incubation Time | 24 hours |
| Result | Caused a significant dose-dependent cytotoxic effect on U2OS cancer cells with an IC50 of 2.1 μM. Induced much weaker growth inhibition on normal BJ fibroblasts with an IC50 of 29.3 μM. |
Apoptosis Analysis[1]
| Cell Line | U2OS cells |
|---|---|
| Concentration | 0.6, 1.2, 2.5 μM |
| Incubation Time | 24 hours |
| Result | Induced significant apoptosis in U2OS cells (the percentage of apoptotic cells increased from 10.1% to 24.9%). |
Cell Cycle Analysis[1]
| Cell Line | U2OS cells |
|---|---|
| Concentration | 0.6, 1.2, 2.5 μM |
| Incubation Time | 24 hours |
| Result | Induced the apparent accumulation of cells in the S phase (increasing from 24.0% to 32.2%) in a dose-dependent manner. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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