| Field | Specification |
|---|---|
| Alternative names | Buthionine sulfoximine hydrochloride; BSO hydrochloride |
| Applications | |
| Molecular weight | |
| Molecular formula | C8H19ClN2O3S |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
DL-Buthionine-(S,R)-sulfoximine hydrochloride, also known as Buthionine sulfoximine hydrochloride and BSO hydrochloride, is a potent inhibitor of glutamylcysteine synthetase biosynthesis. It is supplied as a white to off-white solid (C8H19ClN2O3S, MW 258.77) at 99.09% purity.
Physical & Chemical Properties
| Molecular Formula | C8H19ClN2O3S |
|---|---|
| Molecular Weight | 258.77 g/mol |
| Purity | 99.09% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | OC(C(N)CCS(CCCC)(=N)=O)=O.[H]Cl |
| Signaling Pathway | Apoptosis |
| Solubility | In Vitro: H2O: 100 mg/mL (386.44 mM; Requires sonication) DMSO: < 1 mg/mL (insoluble or slightly soluble) |
| Storage | -20°C, sealed storage, away from moisture and light. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| H2O | 100 mg/mL (386.44 mM) | requires sonication |
| DMSO | < 1 mg/mL | insoluble or slightly soluble |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture and light; avoid repeated freeze-thaw cycles.
If water is used as the stock solvent, dilute to the working solution and sterilize it through a 0.22 μm filter before use.
In Vivo
Choose the formulation that suits the animal model and route of administration. Percentages are volume ratios of the final working solution. Prepare the working solution fresh on the day of dosing; if precipitation or phase separation occurs, gentle warming or sonication can help.
Direct preparation of the working solution
These formulations are prepared directly, without a DMSO stock; use them promptly after preparation.
Protocol 1
| Composition | PBS |
|---|---|
| Result | 100 mg/mL (386.44 mM); clear solution; requires sonication |
Data provided by the manufacturer.
In Vitro
Buthionine sulfoximine, an analog of methionine sulfoximine, inhibits gamma-glutamylcysteine synthetase roughly 20 times more effectively than does prothionine sulfoximine, and it is at least 100 times more effective than methionine sulfoximine[1].
In Vivo
In mice bearing HT1080 and HT1080/DR4 xenografts, nontoxic doses of D,L-Buthionine-(S,R)-sulfoximine (300 and 600 mg/kg/day), given as a continuous i.v infusion, lower GSH plasma levels by 60% and GSH tumor levels by more than 95 %, in both parental and multidrug-resistant tumors[2].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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Catabolism of extracellular glutathione supplies cysteine to support tumours. Nature 2026 May;653(8115):933-941. PMID: 41851454
The Acetyltransferase ARD1 Induces Glutathione Synthesis to Facilitate Ferroptosis Evasion in Hepatocellular Carcinoma. Cancer Res 2025 Aug 21. PMID: 40838989
Catalytic Proximal Protein Oligomerization as an Anti-Tumor Strategy Targeting WDR5. Nat Commun 2026 Mar 13;17(1):3879. PMID: 41820364
SLC13A3 is a major effector downstream of activated β-catenin in liver cancer pathogenesis. Nat Commun 2024 Aug 30;15(1):7522. PMID: 39215042
Suppression of the LKB1-AMPK-SLC7A11-GSH signaling pathway sensitizes NSCLC to albumin-bound paclitaxel via oxidative stress. Redox Biol 2025 Apr:81:103567. PMID: 40023979
NRF2 preserves genomic integrity by facilitating ATR activation and G2 cell cycle arrest. Nucleic Acids Res 2020 Sep 18;48(16):9109-9123. PMID: 32729622
Donafenib and GSK-J4 Synergistically Induce Ferroptosis in Liver Cancer by Upregulating HMOX1 Expression. Adv Sci (Weinh) 2023 Aug;10(22):e2206798. PMID: 37330650
Direct recruitment of TONSOKU by the N-terminal tails of histone variants H2A.X and H2A.W coordinates DNA repair. Sci Adv 2025 Dec 19;11(51):eady2104. PMID: 41406205
Liver regeneration-associated hepatocellular YAP1 activation prevents colorectal cancer liver metastasis through glutamine competition. Sci Adv 2025 Aug 22;11(34):eadw6926. PMID: 40845109
Targeting polyamine metabolism induces oxidative/carbonyl stress to reinvigorate antitumor immunity in prostate cancer. J Control Release 2025 Sep 30;388(Pt 1):114283. PMID: 41038348
